Delivery and quantification of hydrogen peroxide generated via cold atmospheric pressure plasma through biological material
Delivery and quantification of hydrogen peroxide generated via cold atmospheric pressure plasma through biological material
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DOI：
10.1088/1361-6463/ab4539
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发表时间：
2019-10
期刊：
影响因子：
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通讯作者：
H. Hathaway;B. Patenall;N. Thet;A. Sedgwick;G. Williams;A. Jenkins;S. Allinson;R. Short
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文献类型：
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作者：
H. Hathaway;B. Patenall;N. Thet;A. Sedgwick;G. Williams;A. Jenkins;S. Allinson;R. Short
等离子体产生的过氧化氢（H2O2）穿越细菌生物膜的能力和随后产生的H2O2的命运进行了研究。用氦驱动的冷大气压等离子体（CAP）射流处理体外模型，所述体外模型包括浸渍有人工伤口流体和不同成熟度的生物膜的纳米多孔膜。定量生物膜下方产生的H2O2浓度。结果表明，等离子体产生的H2O2与生物膜显着相互作用，从而表现出降低的浓度在整个底层的纳米多孔膜。生物膜的成熟度表现出显着的影响，过氧化氢的渗透深度，这表明，良好的建立，多层生物膜可能会提供一个屏蔽效果相对于位于较低层的生物膜的细胞，从而使他们不太容易受到等离子体消毒。这可能在慢性深部组织感染（如糖尿病和静脉性腿部溃疡）的血浆治疗中具有临床意义。我们的研究结果进行了讨论的背景下，等离子体生物膜相互作用，相对于由CAP，如H2O2产生的较长寿命的活性物种的命运。
The ability of plasma-generated hydrogen peroxide (H2O2) to traverse bacterial biofilms and the subsequent fate of the generated H2O2 has been investigated. An in vitro model, comprising a nanoporous membrane impregnated with artificial wound fluid and biofilms of varying maturity was treated with a helium-driven, cold atmospheric pressure plasma (CAP) jet. The concentration of H2O2 generated below the biofilms was quantified. The results showed that the plasma-generated H2O2 interacted significantly with the biofilm, thus exhibiting a reduction in concentration across the underlying nanoporous membrane. Biofilm maturity exhibited a significant effect on the penetration depth of H2O2, suggesting that well established, multilayer biofilms are likely to offer a shielding effect with respect to cells located in the lower layers of the biofilm, thus rendering them less susceptible to plasma disinfection. This may prove clinically significant in the plasma treatment of chronic, deep tissue infections such as diabetic and venous leg ulcers. Our results are discussed in the context of plasma-biofilm interactions, with respect to the fate of the longer lived reactive species generated by CAP, such as H2O2.