Inherent pacemaker function of duodenal GIST.
Inherent pacemaker function of duodenal GIST.
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DOI：
10.1016/j.ejca.2005.09.024
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发表时间：
2006
影响因子：
8.4
通讯作者：
Shinji Furuzono;S. Ohya;S. Inoue;A. Nakao;Y. Imaizumi;S. Nakayama
中科院分区：
文献类型：
--
作者：
Shinji Furuzono;S. Ohya;S. Inoue;A. Nakao;Y. Imaizumi;S. Nakayama
胃肠道间质瘤(GIST)被认为起源于Cajal间质细胞(ICCs)，ICCs被认为是肠道运动的起搏细胞。分离的细胞是通过酶处理具有频繁功能获得基因突变的人十二指肠GIST组织获得的。细胞培养后，c-Kit免疫反应被保留，细胞突起较长。全细胞膜片钳记录显示电压依赖的外向电流，没有瞬时内向电流。细胞内Ca~(2+)测量显示部分GIST细胞呈现振荡样自发活动。RT-PCR显示离子通道(Kv1.1、Kv1.6和KCNH2；IP3R1和IP3R2；TRPC1、3、6和7；Cx43)的表达，它们被认为在起搏器活动中发挥重要作用。然而，SCN5A，一种已知在人ICCs中表达的抗TTX的Na+通道，低于可检测到的水平。这些数据表明，GIST细胞似乎保留了ICC起搏器活动的部分但不是全部离子机制。
Gastrointestinal stromal tumours (GIST) are thought to derive from interstitial cells of Cajal (ICCs), which are putative pacemaker cells for gut motility. Isolated cells were obtained by enzymatic treatment of human duodenum GIST tissue having a frequent gain-of-function gene mutation. After cell culturing, c-Kit immunoreactivity was preserved and the cells developed long processes. Whole cell patch clamp recordings revealed voltage-dependent outward currents, without transient inward currents. Intracellular Ca2+measurements showed oscillation-like spontaneous activity in some GIST cells. RT-PCR revealed expression of ion channels (Kv1.1, Kv1.6 and KCNH2; IP3R1, and IP3R2; TRPC1, 3, 6 and 7; Cx43), which have been suggested to play important roles in pacemaker activity. However, SCN5A, a TTX-resistant Na+channel known to be expressed in human ICCs, was below detectable levels. These data suggest that GIST cells appear to preserve some, but not all ionic mechanisms underlying pacemaker activity in ICC.