Regulation and characterization of a newly deduced cell wall hydrolase gene (cwlJ) which affects germination of Bacillus subtilis spores
Regulation and characterization of a newly deduced cell wall hydrolase gene (cwlJ) which affects germination of Bacillus subtilis spores
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DOI：
10.1128/jb.180.6.1375-1380.1998
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发表时间：
1998-03-01
影响因子：
3.2
通讯作者：
Sekiguchi, J
中科院分区：
文献类型：
--
作者：
Ishikawa, S;Yamane, K;Sekiguchi, J
枯草芽孢杆菌ycbQ（已更名为cwlJ）的预测氨基酸序列与B的特异性皮层水解酶SleBs的C-末端催化结构域具有高度的相似性。cereus和推导出的B. cwlJ基因的β-半乳糖苷酶活性、北方杂交和引物延伸分析结果表明，cwlJ基因由Eo（E）RNA聚合酶转录，cwlJ基因缺失的芽孢对L-丙氨酸和AGFK均有反应，孢子悬浮液的A（580）值下降比野生型菌株慢，在萌发过程中释放的吡啶二羧酸比野生型菌株少。枯草芽孢杆菌sleB孢子不释放显著水平的己糖胺。虽然cwlJ和sleB孢子能够萌发，但CJSB（cwlJ sleB）孢子不能萌发，但表现出初始萌发反应，例如，A（580）部分降低，吡啶二羧酸缓慢释放，CJSB孢子在萌发液中6 h后变为浅灰色，但其萌发活性远高于sleB突变体孢子。本文还讨论了sleB和cwlJ突变体在孢子萌发和成熟中的作用。
The predicted amino acid sequence of Bacillus subtilis ycbQ (renamed cwlJ) exhibits high similarity to those of the deduced C-terminal catalytic domain of SleBs, the specific cortex-hydrolyzing enzyme of B. cereus and the deduced one of B. subtilis, We constructed a cwlJ::lacZ fusion in the B, subtilis chromosome, The beta-galactosidase activity and results of Northern hybridization and primer extension analyses of the cwlJ gene indicated that it is transcribed by Eo(E) RNA polymerase, cwlJ-deficient spores responded to both L-alanine and AGFK, the A(580) values of spore suspensions decreased more slowly than in the case of the wild-type strain, and the mutant spores released less dipicolinic acid than did those of the wild-type strain during germination, However, the mutant spores released only slightly less hexosamine than did the wild-type spores, In contrast, B. subtilis sleB spores did not release hexosamine at a significant level. While cwlJ and sleB spores were able to germinate, CJSB (cwlJ sleB) spores could not germinate but exhibited initial germination reactions, e.g., partial decrease in A(580), and slow release of dipicolinic acid, CJSB spores became slightly gray after 6 h in the germinant, but their refractility was much greater than that of sleB mutant spores, The roles of the sleB and cwlJ mutations in germination and spore maturation are also discussed.