Integrating hydrodynamic and acoustic cell separation in a hybrid microfluidic device: a numerical analysis
Integrating hydrodynamic and acoustic cell separation in a hybrid microfluidic device: a numerical analysis
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DOI：
10.1007/s00707-022-03206-6
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发表时间：
2022-04-27
期刊：
影响因子：
2.7
通讯作者：
Shamloo, Amir
中科院分区：
文献类型：
--
作者：
Ashkezari, Amir Hossein Kazemipour;Dizani, Mahdi;Shamloo, Amir
细胞分离微流控装置已经发展成为众多的生物医学和临床研究。尽管如此，在实现从异质样品中优异分离靶细胞的方式中仍然存在许多关键问题。与混合微流体方法相关的大量实验研究平行，很容易察觉到缺乏数值研究以优化分离过程及其准确性。在这项研究中，第一次尽我们所知，一个混合系统，通过整合声电泳和夹流分馏（PFF），提出了实现一个可行的系统，广泛的，精确的分离。采用超声场将导致传统PFF微通道中分离距离的显着增强。这种主动-被动方法提供了许多优点，并消除了每种方法的局限性。声学细胞分离中的一个明显缺陷是驱动电压的限制量，因为高电压对生物细胞的影响是不可修复的。另一方面，在PFF方法中，不能获得具有完美分辨率的颗粒分离。在这项工作中提出的新设备根除了上述问题，并提供了一个准确的，连续的分离与较低的要求的RF信号幅度相比，与传统的设备。结果表明，在总流速为5 μ l/min时，仅用2 V就可获得高分辨率的分离。
Cell separation microfluidic devices have evolved into a multitude of biomedical and clinical research. Nonetheless, many critical issues remain in the way of achieving an excellent separation of target cells from a heterogeneous sample. Parallel to the abundant experimental studies related to the hybrid microfluidic methods, it is easy to perceive the lack of numerical investigations in order to optimize the separation process and its accuracy. In this study, for the first time to the best of our knowledge, a hybrid system by integrating acoustophoresis and pinched-flow fractionation (PFF) is proposed to achieve a viable system for a wide-range, precise separation. Employing the ultrasound field would result in remarkably enhanced amplification of separation distance in the conventional PFF microchannels. This active-passive method provides many advantages and expunges the limitations of each method. A noticeable flaw in the acoustic cell separation is the confined amount of driving voltage because of the irreparable effects of high-voltage on the biological cells. On the other hand, in the PFF method, particle separation with a perfect resolution cannot be attained. The novel device presented in this work eradicates the aforementioned issues and provides an accurate, continuous separation with a lower demanded RF signal amplitude in comparison with the conventional devices. The results show that a high-resolution separation can be attained by applying only 2 V to the system with a total flow rate of 5 mu l/min.