Inactivation of mdoH leads to increased expression of colanic acid capsular polysaccharide in Escherichia coli
Inactivation of mdoH leads to increased expression of colanic acid capsular polysaccharide in Escherichia coli
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DOI：
10.1128/jb.179.21.6858-6861.1997
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发表时间：
1997-11-01
影响因子：
3.2
通讯作者：
Trempy, JE
中科院分区：
文献类型：
--
作者：
Ebel, W;Vaughn, GJ;Trempy, JE
在大肠杆菌lon（+）细胞中通常以低水平表达的荚膜基因（cps）表达，在携带Tn10：：Delta kan插入的lon（+）细胞中增加了38倍，在E. coli染色体。rcsA、rcsB或rcsC中的突变消除了Tn10：：Delta kan插入的效应。对Tn10：：Delta kan插入的两侧进行测序，将插入定位于先前报道的mdoH基因，该基因编码一种参与膜衍生寡糖（MDO）生物合成的蛋白质。提出了一个模型，表明MDO的周质水平的行为，以信号RcsC激活cps的表达。
Capsule gene (cps) expression, which normally occurs at low levels in Escherichia coli lon(+) cells, increased 38-fold in lon(+) cells carrying a Tn10::Delta kan insertion mapping to 24 min on the E. coli chromosome. Null mutations in rcsA, rcsB, or rcsC abolished the effect of the Tn10::Delta kan insertion. Sequencing of both sides of the Tn10::Delta kan insertion localized the insertion to the previously reported mdoH gene, which encodes a protein involved in biosynthesis of membrane-derived oligosaccharides (MDOs). A model suggesting that the periplasmic levels of MDOs act to signal RcsC to activate cps expression is proposed.