Seawater acidification aggravated cadmium toxicity in the oyster Crassostrea gigas: Metal bioaccumulation, subcellular distribution and multiple physiological responses
Seawater acidification aggravated cadmium toxicity in the oyster Crassostrea gigas: Metal bioaccumulation, subcellular distribution and multiple physiological responses
复制标题
DOI：
10.1016/j.scitotenv.2018.06.126
复制
发表时间：
2018-11-15
影响因子：
9.8
通讯作者：
Zhao, Jianmin
中科院分区：
文献类型：
--
作者：
Cao, Ruiwen;Liu, Yongliang;Zhao, Jianmin
越来越多的证据表明，海洋酸化和其他环境压力因素对海洋生物的综合影响。虽然金属污染在海岸和河口广泛分布，但直到最近几年，OA和金属污染的联合效应才受到重视。在本研究中，研究了单独或联合暴露于OA和Cd 31天后，牡蛎长牡蛎的镉（Cd）积累和亚细胞分布以及生理反应。在较高的pCO(2)暴露下，双鱼的鳃（pH值为7.8时增加57%，pH值为7.6时增加22%）和消化腺（pH值为7.8.22%，pH值为7.6时增加38%）的Cd积累均有所增加。虽然在牡蛎鳃和消化腺中发现了类似的总Cd积累模式，但Cd暴露处理中，与消化腺（约45%）相比，在牡蛎鳃的BIM（生物活性金属）部分中发现了更高的Cd分割（约60%），这可能与鳃中普遍较低的毒性相对应。此外，本研究还观察到Cd和OA对暴露牡蛎的氧化应激、组织病理学损伤和凋亡的协同作用，这可能是这两个因素在ROS生成增加方面的显著相互作用。这些研究结果表明，OA会加重海洋生物中金属的毒性，对沿海底栖生态系统产生重大影响，因为金属污染广泛存在，同时酸化海水增加。(C) 2018 Elsevier B.V.版权所有
Mounting evidence has demonstrated the combined effects of ocean acidification (OA) and other environmental stressors on marine organisms. Although metal pollution is widely distributed in coasts and estuaries, the combined effects of OA and metal pollution have received little attention until recent years. In this study, the accumulation and subcellular distribution of cadmium (Cd) and the physiological responses of the oyster Crassostrea gigas were investigated after 31 days of exposure to OA and Cd, either alone or in combination. Increased Cd accumulation was found both in gills (about 57% increase at pH 7.8, 22% increase at pH 7.6) and digestive glands (about 38% increase at pH 7.8.22% increase at pH 7.6) of C. gigas under elevated pCO(2) exposure. Although a similar total Cd accumulation pattern was seen in oyster gills and digestive glands, a higher partition of Cd in the BIM (biologically inactive metal) fractions of gills (about 60%) was found in Cd-exposed treatments compared to the digestive glands (about 45%), which might correspond to the generally lower toxicity in gills. Moreover, synergetic effects of Cd and OA on the oxidative stresses, histopathological damage, and apoptosis of exposed oysters were observed in this study, which might be explained by significant interactions of these two factors on increased generation of ROS. These findings demonstrated that OA could aggravate the toxicity of metals in marine organisms, with significant implications for coastal benthic ecosystems regarding the widespread metal contamination and the concurrent increase of acidified seawater. (C) 2018 Elsevier B.V. All rights reserved.