Independent positioning of microelectrodes for multisite recordings in vitro
Independent positioning of microelectrodes for multisite recordings in vitro
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DOI：
10.1016/j.jneumeth.2008.09.001
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发表时间：
2009-01-30
影响因子：
3
通讯作者：
Heinemann, Uwe
中科院分区：
文献类型：
--
作者：
Albus, Klaus;Sinske, Kurt;Heinemann, Uwe
描述了一种坚固且易于操作、廉价的多站点记录系统，该系统允许独立定位多个微电极，并且每个电极具有高精度的轴向移动。该系统的基本单元是用于左右和上下移动的手动操纵器和用于在千分尺轴方向上定位的千分尺。千分尺由独立控制器模块操作的直流电机驱动，允许以步进模式或连续模式远程控制电机。多站点记录系统已被证明可以稳定地同时记录大鼠器官型海马切片培养物 (OHSC) 中的单个单位和群体活动、细胞外离子浓度和细胞内电位。 (C) 2008 Elsevier B.V. 保留所有权利。
A robust and easy to handle, inexpensive multisite recording system is described which allows independent positioning of several microelectrodes with high precision axial movement of each electrode. The basic units of the system are a manually operated manipulator for left-right and up-down movement and a micrometer for positioning in the direction of the micrometer axis. The micrometer is actuated with a DC-motor operated by a stand-alone controller module allowing for remote control of the motor in either step mode or continuous mode. The multisite recording system has been proven to allow stable simultaneous recordings of single unit and population activities, extracellular ion concentrations and intracellular potentials in organotypic hippocampal slice cultures (OHSCs) of rat. (C) 2008 Elsevier B.V. All rights reserved.