Ribonucleic acid release activity of transcription termination protein rho is dependent on the hydrolysis of nucleoside triphosphates.
Ribonucleic acid release activity of transcription termination protein rho is dependent on the hydrolysis of nucleoside triphosphates.
复制标题
转录终止蛋白rho的核糖核酸释放活性依赖于三磷酸核苷的水解。
DOI：
10.1021/bi00559a022
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发表时间：
1980
期刊：
影响因子：
2.9
通讯作者：
Conaway,R
中科院分区：
文献类型：
--
作者：
Richardson,JP;Conaway,R
John P. Richardson* 和罗纳德Conaway* 摘要：设计了一种测定方法来测量RNA链从转录复合物中的p依赖性释放。结果表明，p刺激RNA从由新生RNA分子、大肠杆菌RNA聚合酶和T7 DNA组成的三元转录复合物中释放，这种刺激依赖于p-NTR的核苷酸底物的存在。几个实验得出的结论是，释放是NTP水解依赖性p作用于RNA的直接结果。首先，作为p-NTP底物的任何核苷酸满足NTP要求，但不被p作用水解的核苷酸不满足。此外，一些激活释放的核苷酸是RNA聚合酶的不良底物，这一事实表明，释放不依赖于进一步向新生链添加核苷酸。第二，释放和RNA-I的反应条件。来自大肠杆菌的NA聚合酶通过进行性机制催化来自双螺旋DNA模板的RNA的聚合（Krakow等人，1976年）。起始后，RNA链不会从其与酶和DNA的复合物中释放出来，直到其合成完全终止。由于核苷酸添加到新生RNA的速率在DNA中的所有序列处都不均匀（Darlix & Fro-mageot，1972; Maizels，1973; Rosenberg等人，1978年），RNA聚合酶会在某些区域暂停，
John P. Richardson* and Ronald Conaway* abstract: An assay is devised to measure p-dependent release of RNA chains from transcription complexes. It is shown that p stimulates therelease of RNA fromisolated ternary tran-scription complexes consisting of nascent RNA molecules, Escherichia coli RNA polymerase, and T7 DNA and that this stimulation is dependent on the presence of a nucleotide substrate for p-NTPase. Several experiments lead to the conclusion that release is a direct consequence of a NTP hy-drolysis dependent p action on the RNA. First, the NTP requirement is satisfied by any of the nucleotides which are substrates for p-NTPase but is not satisfied by nucleotides that are not hydrolyzed by p action. Furthermore, the fact that some of the nucleotides that do activate release are poor substrates for RNA polymerase suggests that release is not dependent upon further nucleotide addition to the nascent chain. Second, the reaction conditions for release and RNA-I^. NA polymerase from Escherichia coli catalyzes the po-lymerization of RNA from a double-helical DNA template by a processive mechanism (Krakow et al., 1976). After initiation, an RNA chain is not released from its complex with the enzyme and the DNA until its synthesis is fully terminated. Since the rate of addition of nucleotides to a nascent RNA is not uniform at all sequences in the DNA (Darlix & Fro-mageot, 1972; Maizels, 1973; Rosenberg et al., 1978), there will be regions where RNA polymerase will pause