SUBTHALAMIC NUCLEUS EVOKES SIMILAR LONG LASTING GLUTAMATERGIC EXCITATIONS IN PALLIDAL, ENTOPEDUNCULAR AND NIGRAL NEURONS IN THE BASAL GANGLIA SLICE
SUBTHALAMIC NUCLEUS EVOKES SIMILAR LONG LASTING GLUTAMATERGIC EXCITATIONS IN PALLIDAL, ENTOPEDUNCULAR AND NIGRAL NEURONS IN THE BASAL GANGLIA SLICE
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DOI：
10.1016/j.neuroscience.2010.01.011
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发表时间：
2010-03-31
期刊：
影响因子：
3.3
通讯作者：
Hammond, C.
中科院分区：
文献类型：
--
作者：
Ammari, R.;Lopez, C.;Hammond, C.
丘脑底核(STN)通过其直接谷氨酸能投射调节苍白球(GP)、内丘脑内侧核(EP)和黑质网状部(SNR)神经元的活动。为了研究STN影响这些结构中活动的机制以及STN诱导的活动在STN靶神经元中是否具有可比性，我们在保留这些功能联系的倾斜的矢状旁基底节切片(BGS)上进行了膜片钳记录。我们报道，单一的、短暂的刺激STN在GP、EP和SNR神经元上产生短暂的单突触AMPA介导的兴奋性突触后电流(EPSC)。较高强度的阈值以上激活可激发复合EPSC，该复合EPSC由早期的单突触成分和随后缓慢的NMDA介导的内向电流以及AMPA介导的EPSCs组成。这些晚期的EPSC是多突触的，并引起持续数百毫秒的尖峰爆发。通过手术将STN从BGS切片上移除，它们被消除，这表明STN是它们产生所必需的。对充满生物细胞素的STN神经元的重建显示，三分之一的STN神经元投射出STN内的轴突侧支，这可能是多突触活动的基础。我们认为，激活STN通过多突触网络在其靶神经元中产生类似的长时间兴奋。(C)2010年IBRO。爱思唯尔有限公司出版。保留所有权利。
The subthalamic nucleus (STN) modulates the activity of globus pallidus (GP), entopeduncular nucleus (EP) and substantia nigra pars reticulata (SNr) neurons via its direct glutamatergic projections. To investigate the mechanism by which STN affects activity in these structures and whether STN induced activity is comparable among STN target neurons, we performed patch clamp recordings in a tilted, parasagittal, basal ganglia slice (BGS) that preserves these functional connections. We report that single, brief stimulation of the STN generates a brief monosynaptic AMPA-mediated excitatory postsynaptic current (EPSC) in GP, EP and SNr neurons. A higher intensity, supra-threshold activation evokes a compound EPSC consisting of an early monosynaptic component followed by a slow inward NMDA-mediated current with an overlying barrage of AMPA-mediated EPSCs. These late EPSCs were polysynaptic and gave rise to bursts of spikes that lasted several hundreds of milliseconds. They were eliminated by surgical removal of the STN from the BGS slice, indicating that the STN is required for their generation. Reconstruction of biocytin-filled STN neurons revealed that a third of STN neurons project intra-STN axon collaterals that may underlie polysynaptic activity. We propose that activation of the STN yields comparable long lasting excitations in its target neurons by means of a polysynaptic network. (C) 2010 IBRO. Published by Elsevier Ltd. All rights reserved.