Molecular mechanism of paramyxovirus budding
Molecular mechanism of paramyxovirus budding
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DOI：
10.1016/j.virusres.2004.08.010
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发表时间：
2004-12-01
期刊：
影响因子：
5
通讯作者：
Portner, A
中科院分区：
文献类型：
--
作者：
Takimoto, T;Portner, A
副粘病毒组分组装在感染细胞的质膜上，子代病毒通过萌发过程形成。虽然驱动发芽的分子机制(膜弯曲和“夹断”反应)还不是很清楚，但病毒基质(M)蛋白被认为在这一过程中发挥了重要作用。M蛋白形成致密的一层，与感染细胞的质膜内小叶紧密相连。一些副粘病毒M蛋白的表达会导致含有M蛋白的病毒样颗粒的形成和释放；因此，在这些病毒中，M蛋白本身显然可以触发病毒样颗粒形成和释放所需的所有步骤。M还与病毒包膜糖蛋白和核衣壳特异地相互作用，并参与将病毒成分定向运输到极化细胞顶端表面的萌发位置。此外，M与病毒糖蛋白胞质尾部、M与核衣壳之间的蛋白质-蛋白质相互作用影响病毒的生产效率。病毒粒子的结构组织和M蛋白的功能清楚地表明，该蛋白协调副粘病毒的萌发。(C)2004爱思唯尔B.V.保留所有权利。
Components of paramyxoviruses are assembled at the plasma membrane of infected cells, and progeny viruses are formed by the budding process. Although the molecular mechanisms that drive budding (membrane curving and "pinching-off" reaction) are not well understood, the viral matrix (M) protein is thought to play a major role in the process. The M protein forms a dense layer tightly associated with the inner leaflet of the plasma membrane of infected cells. Expression of the M protein of some paramyxoviruses results in the formation and release of virus-like particles that contain the M protein; thus, in these viruses, the M protein alone can apparently trigger all steps required for the formation and release of virus-like particles. M also interacts specifically with viral envelope glycoproteins and nucleocapsids and is involved in directed transport of viral components to the budding site at the apical surface of polarized cells. In addition, protein-protein interactions between M and the cytoplasmic tail of viral glycoproteins and between M and the nucleocapsid affect the efficiency of virus production. The structural organization of the virion and the functions of the M protein clearly indicate that this protein orchestrates the budding of paramyxovirus. (C) 2004 Elsevier B.V. All rights reserved.