RNA and protein localisations of TGF beta 2 in the early mouse embryo suggest an involvement in cardiac development.
RNA and protein localisations of TGF beta 2 in the early mouse embryo suggest an involvement in cardiac development.
复制标题
DOI：
--
复制
发表时间：
1993-02
期刊：
影响因子：
4.6
通讯作者：
M. Dickson;H. Slager;E. Duffie;C. Mummery;R. Akhurst
中科院分区：
文献类型：
--
作者：
M. Dickson;H. Slager;E. Duffie;C. Mummery;R. Akhurst
我们已经进行了详细的分析，从6.5至9.5天后，小鼠胚胎中的TGF β 2和β 3的RNA和TGF β 2蛋白的定位，使用原位杂交和免疫组织化学连续切片，和整体安装原位杂交，以完成胚胎。在任何组织切片中均未观察到TGF β 3 RNA，但在心脏移植后8.5天，通过心脏流出道周围的整体原位杂交观察到非常低水平的RNA。TGF β 2 RNA在所有具有分化为心肌细胞潜力的细胞中均以高水平表达。此外，与心脏并置的前肠内胚层和前肠嘴端的神经上皮细胞在结肠切除后8.5和9.5天之间表达非常高水平的TGF β 2 RNA。随着心肌形成的进行，TGF β 2 RNA水平在肌细胞内减少，伴随着TGF β 2蛋白染色的增加。心肌细胞的TGF β 2蛋白染色在整个发育过程中持续存在，并且在成人中，在相应RNA的可检测水平的情况下。叠加在这种心肌表达模式上，在心脏停搏后8.5和9.5天之间，流出道和房室管的心肌中存在TGF β 2 RNA的上调，其在心脏停搏后11.5天恢复到低水平。结果进行了讨论的TGF β 2在控制心肌发生和诱导相互作用，导致心脏垫组织形成的潜在作用。
We have performed a detailed analysis of the localisations of RNAs for TGF beta 2 and beta 3, and of TGF beta 2 protein in mouse embryos from 6.5 to 9.5 days post coitum, using in situ hybridisation and immunohistochemistry on serial sections, and whole-mount in situ hybridisation to complete embryos. TGF beta 3 RNA was not seen in any of the tissue sections, but very low levels of the RNA were seen by whole-mount in situ hybridisation around the outflow tract of the heart at 8.5 days post coitum. TGF beta 2 RNA is expressed at high levels in all cells with the potential to differentiate into cardiomyocytes. Additionally, the foregut endoderm, juxtaposed to the heart, and the neuroepithelium at the rostral extremity of the foregut, express very high levels of TGF beta 2 RNA, between 8.5 and 9.5 days post coitum. As cardiomyogenesis proceeds, TGF beta 2 RNA levels diminishes within the myocytes, with a concomitant increase in staining for TGF beta 2 protein. TGF beta 2 protein staining of cardiomyocytes persists throughout development and in the adult, in the absence of detectable levels of the corresponding RNA. Superimposed upon this myocardial pattern of expression, there is an upregulation of TGF beta 2 RNA in the myocardium of the outflow tract and atrioventricular canal between 8.5 and 9.5 days post coitum, which returns to low levels by 11.5 days post coitum. The results are discussed in terms of a potential role of TGF beta 2 in controlling cardiomyogenesis and in inductive interactions leading to cardiac cushion tissue formation.