Fibrillar assemblage of variable segments of immunoglobulin light chains: an electron microscopic study.
Fibrillar assemblage of variable segments of immunoglobulin light chains: an electron microscopic study.
复制标题
免疫球蛋白轻链可变片段的纤维状组装：电子显微镜研究。
作者：
T. Shirahama;M. Benson;A. Cohen;A. Tanaka
将本氏蛋白酶切为可变片段和恒定片段，然后在中性pH下沉淀可变片段。沉淀物用刚果红染色良好，并且在这种染色后在偏振光下显示绿色双折射。高分辨电子显微镜的阴影铸造和负染色的准备工作表明，它们包括三种类型的纤维结构，分别测量10至20微米，25至40微米和75至100微米的宽度。较薄的纤维状结构占主导地位的样品从沉淀过程的早期阶段，而较厚的占主导地位的后期样品。这些结构的连续出现和其他观察结果表明，它们代表了不同发育阶段的原纤维。沉淀物的这些光镜和电镜方面有许多与淀粉样蛋白相对应的点，尽管这两种物质之间的同一性仍有待确定。此外，纤维状结构可以产生在13个本琼斯蛋白以相同的方式处理只有3。
Bence Jones protein was enzymatically cleaved into its variable and constant segments, and then the variable segment was precipitated at neutral pH. The precipitate stained well with Congo red and showed green birefringence under the polarized light after such staining. High resolution electron microscopy of shadow-casted and negatively stained preparations revealed that they consisted of three types of fibrillar structures, respectively measuring 10 to 20 Å, 25 to 40 Å and 75 to 100 Å in width. The thinner fibrillar structures dominated the specimens from earlier stages of the precipitation procedure, while thicker ones predominated in the later samples. The sequential appearance of these structures and other observations suggested that they represented fibrils in different stages of development. These light and electron microscopic aspects of the precipitate have many points corresponding with those of the amyloid, although the identity between the two substances remains to be established. Moreover, the fibrillar structures could be produced in only 3 of 13 Bence Jones proteins treated in an identical fashion.