Focal adhesion kinase as well as p130Cas and paxillin is crucially involved in the enhanced malignant properties under expression of ganglioside GD3 in melanoma cells
Focal adhesion kinase as well as p130Cas and paxillin is crucially involved in the enhanced malignant properties under expression of ganglioside GD3 in melanoma cells
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DOI：
10.1016/j.bbagen.2007.11.002
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发表时间：
2008-03-01
影响因子：
3
通讯作者：
Furukawa, Keiko
中科院分区：
文献类型：
--
作者：
Hamamura, Kazunori;Tsuji, Momoko;Furukawa, Keiko
用PY20（抗磷酸酪氨酸抗体）对来自血清处理的表达GD3的黑素瘤细胞的免疫沉淀物进行质谱分析，结果显示，与GD3阴性细胞相比，在表达GD3的细胞中粘着斑激酶（FAK）被更强烈地激活。通过siRNA介导的敲除证实了FAK参与表达GD3的黑素瘤的增殖和侵袭增加。此外，研究表明FAK在增强的信号通路中位于p130Cas和桩蛋白的上游。GD3表达增强了胎牛血清处理后FAK与p130Cas的结合。因此，黏着斑激酶以及p130Cas和桩蛋白应该是一个关键的分子经历更强的酪氨酸磷酸化GD3表达黑色素瘤细胞。连接GD3和FAK的分子如增强信号通路中的整合素仍有待研究。(c)2007 Elsevier B.V.保留所有权利。
Mass spectrometry analysis of immunoprecipitates from serum-treated GD3-expressing melanoma cells with PY20 (anti-phosphotyrosine antibody) revealed that focal adhesion kinase (FAK) is more strongly activated in GD3-expressing cells than in GD3-negative cells. Involvent of FAK in the increased proliferation and invasion in GD3-expressing melanomas was demonstrated by siRNA-mediated knockdown. Also, it was shown that FAK is located up-stream of p130Cas and paxillin in the enhanced signaling pathway. GD3 expression enhanced the association of FAK with p130Cas after treatment with fetal calf serum. Thus, focal adhesion kinase as well as p130Cas and paxillin should be a crucial molecule undergoing stronger tyrosine phosphorylation in GD3-expressing melanoma cells. Molecules linking GD3 and FAK such as integrins in the enhanced signaling pathway remain to be investigated. (c) 2007 Elsevier B.V. All rights reserved.