Deformation Response of Catenated DNA Networks in a Planar Elongational Field
Deformation Response of Catenated DNA Networks in a Planar Elongational Field
复制标题
平面伸长场中串联 DNA 网络的变形响应
DOI：
10.1021/acsmacrolett.0c00360
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发表时间：
2020
影响因子：
7.015
通讯作者：
Doyle, Patrick S.
中科院分区：
文献类型：
--
作者：
Soh, Beatrice W.;Doyle, Patrick S.
动丝泡体是一种复杂的链状DNA网络，类似于二维聚合系统。在这项工作中，我们使用单分子实验来研究动质粒在平面伸长场中的瞬时和稳态变形。我们证明，由于中间亚稳态的构象重排，动胞体以阶段性方式变形，并在大应变下经历瞬时变形。动泡体在拉伸场中实现依赖于应变率的稳态变形，类似于线性聚合物的变形。与线形聚合物的卷曲-拉伸转变不同，我们没有观察到动泡体的非变形状态和变形状态之间的突然转变。
A kinetoplast is a complex catenated DNA network that bears resemblance to a two-dimensional polymeric system. In this work, we use single-molecule experiments to study the transient and steady-state deformation of kinetoplasts in a planar elongational field. We demonstrate that kinetoplasts deform in a stagewise manner and undergo transient deformation at large strains, due to conformational rearrangements from an intermediate metastable state. Kinetoplasts in an elongational field achieve a steady-state deformation that depends on strain rate, akin to the deformation of linear polymers. We do not observe an abrupt transition between the nondeformed and deformed states of a kinetoplast, in contrast to the coil–stretch transition for a linear polymer.