Maternal-fetal interaction: preconception immunization in mice prevents neonatal sensitization induced by allergen exposure during pregnancy and breastfeeding
Maternal-fetal interaction: preconception immunization in mice prevents neonatal sensitization induced by allergen exposure during pregnancy and breastfeeding
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DOI：
10.1111/j.1365-2567.2007.02618.x
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发表时间：
2007-09-01
期刊：
影响因子：
6.4
通讯作者：
Sato, Maria N.
中科院分区：
文献类型：
--
作者：
Fusaro, Ana E.;Brito, Cyro A.;Sato, Maria N.
妊娠期和哺乳期的过敏原排除措施在初级过敏预防研究中已被考虑，但完全避免母亲/新生儿接触过敏原已被证明是一个困难的过程。为了评估在小鼠生命早期预防过敏原致敏的策略，我们首先建立了一个新生儿模型，通过母体在怀孕或哺乳期间暴露过敏原致敏。研究了孕前免疫对新生儿对母体过敏原暴露的后续过敏反应的调节潜力。本研究表明，在怀孕或哺乳期间暴露于抗原的免疫母亲发生了强烈的抗体垂直传播，包括免疫球蛋白G （IgG）与卵清蛋白和具有过敏功能的IgG1抗体复合物。进一步表明，母体免疫有效地减少了母乳喂养中游离抗原的传代，抑制了出生后抗原暴露后增强的IgE抗体反应。此外，产前免疫降低了免疫新生儿的抗原特异性增殖反应，同时对非特异性刺激后T细胞和B细胞的激活和分化以及细胞因子的产生都有深远的下调作用。这些发现表明，母亲在产前和产后暴露于过敏原后的早期生活敏化可以通过母亲的预防性疫苗接种来避免。
Allergen exclusion measures during pregnancy and lactation have been given consideration in studies of primary allergy prevention but complete avoidance of mother/neonatal allergen exposure has proven to be a difficult procedure. To evaluate a strategy to prevent allergen sensitization in early life in mice, we first established a neonatal model with ovalbumin sensitization through maternal allergen exposure during pregnancy or breastfeeding. The modulatory potential of preconception immunization was investigated on the neonatal development of subsequent allergic responses to maternal allergen exposure. Herein, we demonstrate that immunized mothers exposed to antigen during pregnancy or breastfeeding underwent intense vertical transmission of antibodies, including immunoglobulin G ( IgG) in complex with ovalbumin and IgG1 antibody with anaphylactic function. It was further shown that maternal immunization efficiently decreased the passage of free antigens through breastfeeding and inhibited the enhanced IgE antibody response after postnatal antigen exposure. In addition, antenatal immunization decreased the antigen-specific proliferative response of immunized neonates, in parallel with profound downmodulatory effects on both the activation and differentiation of T and B cells after a non-specific stimulus and cytokine production. These findings showed that early life sensitization, subsequent to maternal allergen exposure during both the prenatal and postnatal periods, could be avoided by preventive vaccination of the mother.