Complete genome sequence of a new orthotospovirus associated with ringspot in Fatsia japonica
Complete genome sequence of a new orthotospovirus associated with ringspot in Fatsia japonica
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与日本金霸王环斑相关的新型正孢病毒的完整基因组序列
DOI：
10.1007/s00705-021-05300-x
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发表时间：
2022
影响因子：
2.7
通讯作者：
Yamaji Yasuyuki
中科院分区：
文献类型：
--
作者：
Kitazawa Yugo;Nijo Takamichi;Nishikawa Masanobu;Matsumoto Oki;Suzuki Takumi;Suzuki Masato;Maejima Kensaku;Namba Shigetou;Yamaji Yasuyuki
日本金针菇（Fatsia japonica）是一种常绿灌木，原产于日本。几十年来，人们在 F. 的叶子上观察到类似病毒的环斑症状。日本粳稻；然而，之前确定致病因素的尝试并未成功。在这项研究中，我们在有症状的 F 中检测到了类似正孢子病毒的序列。使用 RNA 测序分析粳稻植物。使用常规测序策略确定病毒的 L、M 和 S 片段的完整核苷酸序列。该病毒具有典型的正孢子病毒基因组结构，假定的核衣壳蛋白与花生褪绿扇斑病毒的序列同一性最高，在氨基酸水平上具有83.7%的同一性（低于正孢子病毒属90%的物种划分截止值）。虽然我们无法确认F中病毒的致病性。粳稻由于机械接种存在困难，仅在有症状的叶区检测到病毒这一事实表明其与观察到的症状存在关联。基于这些结果，我们认为这种病毒（我们将其命名为“Fatsia japonica环斑相关病毒”（FjRSaV））是新的正孢病毒属的第一个代表，为此我们提出了二项式“正孢病毒”。
Fatsia japonicais an evergreen shrub native to Japan. For decades, virus-like ringspot symptoms have been observed on leaves ofF. japonicain Japan; however, previous attempts to identify the causal agents have been unsuccessful. In this study, we detected an orthotospovirus-like sequence in symptomaticF. japonicaplants using RNA sequencing analysis. The complete nucleotide sequences of the L, M, and S segments of the virus were determined using conventional sequencing strategies. The virus had a typical orthotospovirus genome structure, and the putative nucleocapsid protein showed the highest sequence identity to that of groundnut chlorotic fan-spot virus, with 83.7% identity at the amino acid level (which is below the 90% species demarcation cutoff for the genusOrthotospovirus). Although we could not confirm the pathogenicity of the virus inF. japonicadue to difficulties associated with mechanical inoculation, its association with the observed symptoms was suggested by the fact that the virus was detected only in symptomatic leaf areas. Based on these results, we consider this virus, which we have named "Fatsia japonica ringspot-associated virus" (FjRSaV), to be the first representative of a new orthotospovirus species, for which we propose the binomial "Orthotospovirus fatsiae".
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影响因子：
10.7
作者：
Kumar, Sudhir;Stecher, Glen;Tamura, Koichiro
通讯作者：
Tamura, Koichiro
从日本盛冈烟草植物中分离的番茄斑萎病毒的纯化
DOI：
10.3186/jjphytopath.57.239
发表时间：
1991
期刊：
Japanese Journal of Phytopathology
影响因子：
--
作者：
S. Tsuda;K. T. Natsuaki;K. Tomaru
通讯作者：
K. Tomaru
从日本明日叶中获得的胡萝卜旋涡病毒 1 号分离株的完整基因组序列
影响因子：
0.8
作者：
Tokuda Ryosuke;Nishikawa Masanobu;Hosoe Naoi;Nijo Takamichi;Iwabuchi Nozomu;Yoshida Tetsuya;Watanabe Kiyoto;Maejima Kensaku;Yamaji Yasuyuki;Namba Shigetou
通讯作者：
Namba Shigetou
影响因子：
5.6
作者：
ALTSCHUL, SF;GISH, W;LIPMAN, DJ
通讯作者：
LIPMAN, DJ