Molecular cloning of prohormone convertase 1 from the atrial gland of Aplysia.
Molecular cloning of prohormone convertase 1 from the atrial gland of Aplysia.
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海兔心房腺激素原转化酶 1 的分子克隆。
DOI：
10.1089/dna.1996.15.339
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发表时间：
1996
影响因子：
3.1
通讯作者：
Kurosky,A
中科院分区：
文献类型：
--
作者：
Gorham,EL;Nagle,GT;Smith,JS;Shen,H;Kurosky,A
我们用聚合酶链反应（PCR）制备的激素原转化酶（PC）1探针筛选了一个心房腺cDNA文库，并分离出一个与AblasiaPC 1相关的全长3.6kb cDNA克隆。该cDNA序列（3，565 bp）编码一个推定的703个氨基酸残基的前内切蛋白酶原（APC 1），其与其他真核生物PC 1具有相当大的序列同一性，并且与一个APC1B具有高度的序列同一性。心房腺RNA的北方印迹分析鉴定了3.9 kb和5.0 kb的APC 1转录物。APC 1是一个候选PC，可能在心房腺分泌细胞中的产卵激素（ELH）相关前体的加工中发挥重要作用，并代表了PC 1在外分泌组织中表达的第一个例子之一。
We have screened anAplysiaatrial gland cDNA library using a prohormone convertase (PC)1 probe prepared by polymerase chain reaction (PCR) and have isolated anAplysiaPC1-related full-length 3.6-kb cDNA clone. The cDNA sequence (3,565 bp) encoded a putative preproendoprotease (APC1) of 703 amino acid residues that showed considerable sequence identity with other eukaryotic PC1s, and indicated a high degree of sequence identity with anAplysianervous system PC sequence (aPC1B). Northern blot analysis of atrial gland RNA identified two APC1 transcripts of 3.9 kb and 5.0 kb. APC1 is a candidate PC that may play an important role in the processing of egg-laying hormone (ELH)-related precursors in atrial gland secretory cells and represents one of the first examples of PC1 expression in an exocrine tissue.