Deregulation of miR-92a expression is implicated in hepatocellular carcinoma development
Deregulation of miR-92a expression is implicated in hepatocellular carcinoma development
复制标题
DOI：
10.1111/j.1440-1827.2010.02526.x
复制
发表时间：
2010-05-01
影响因子：
2.2
通讯作者：
Kuroda, Masahiko
中科院分区：
文献类型：
--
作者：
Shigoka, Masatoshi;Tsuchida, Akihiko;Kuroda, Masahiko
MicroRNAs （miRNAs）是一类内源表达的非编码小rna，其主要功能是基因调控。越来越多的证据表明，mirna在肿瘤发展中发挥着重要作用，可能成为癌症诊断和预后的强大生物标志物。特别是miR-17-92簇在几种癌症中明显过表达，并与癌症的发生和进展有关。在这项研究中，我们已经证明miR-92a在肝细胞癌（HCC）中高表达。此外，miR-92a可增强hcc来源细胞系的增殖，抗miR-92a拮抗剂可抑制其增殖。另一方面，我们发现HCC患者血浆中miR-92a的相对含量与健康供者相比有所降低。有趣的是，手术治疗后miR-92a的含量升高。因此，尽管血浆中miR-92a水平降低的生理意义尚不清楚，但细胞和血浆中miR-92表达的失调可能与HCC的发生有关。
MicroRNAs (miRNAs) belong to a class of the endogenously expressed non-coding small RNAs which primarily function as gene regulators. Growing evidence suggests that miRNAs have a significant role in tumor development and may constitute robust biomarkers for cancer diagnosis and prognosis. The miR-17-92 cluster especially is markedly overexpressed in several cancers, and is associated with the cancer development and progression. In this study, we have demonstrated that miR-92a is highly expressed in hepatocellular carcinoma (HCC). In addition, the proliferation of HCC-derived cell lines was enhanced by miR-92a and inhibited by the anti-miR-92a antagomir. On the other hand, we have found that the relative amount of miR-92a in the plasmas from HCC patients is decreased compared with that from the healthy donors. Interestingly, the amount of miR-92a was elevated after surgical treatment. Thus, although the physiological significance of the decrease of miR-92a in plasma is still unknown, deregulation of miR-92 expression in cells and plasma should be implicated in the development of HCC.