Magnetic resonance imaging of pulmonary lesions in guinea pigs infected with Mycobacterium tuberculosis
Magnetic resonance imaging of pulmonary lesions in guinea pigs infected with Mycobacterium tuberculosis
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DOI：
10.1128/iai.72.10.5963-5971.2004
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发表时间：
2004-10-01
影响因子：
3.1
通讯作者：
Basaraba, RJ
中科院分区：
文献类型：
--
作者：
Kraft, SL;Dailey, D;Basaraba, RJ
我们利用磁共振成像可视化病变的肺感染豚鼠低剂量气溶胶暴露于结核分枝杆菌。病变在这些图像中很突出，彩色三维重建图像显示肺内分布非常均匀。1个月后的病变数量与气溶胶暴露算法大致相似，表明每个都是由单一细菌建立的。第一个月内，未保护动物和接种动物的病变数量相似，但随后对照动物的病变数量增加，表明发生了继发性病变。对照组豚鼠的病变大小逐渐增加，而卡介苗接种组动物的病变仍然很小。未受保护动物的疾病病理学的一个突出特征是纵隔淋巴结簇的快速和严重的淋巴结病，考虑到此时细胞免疫的强状态，这是矛盾的。这种技术方法的进一步发展可能在寻找新的结核病疫苗时跟踪病变大小、数量和进展方面非常有用。
We utilized magnetic resonance imaging to visualize lesions in the lungs of guinea pigs infected by low-dose aerosol exposure to Mycobacterium tuberculosis. Lesions were prominent in such images, and colorized three-dimensional reconstructions of images revealed a very uniform distribution in the lungs. Lesion numbers after 1 month were approximately similar to the aerosol exposure algorithm, suggesting that each was established by a single bacterium. Numbers of lesions in unprotected and vaccinated animals were similar over the first month but increased thereafter in the control animals, indicating secondary lesion development. Whereas lesion sizes increased progressively in control guinea pigs, lesions remained small in BCG-vaccinated animals. A prominent feature of the disease pathology in unprotected animals was rapid and severe lymphadenopathy of the mediastinal lymph node cluster, which is paradoxical given the strong state of cellular immunity at this time. Further development of this technical approach could be very useful in tracking lesion size, number, and progression in the search for new tuberculosis vaccines.