Emergence of the community-acquired methicillin-resistant Staphylococcus aureus USA300 clone in a Japanese child, demonstrating multiple divergent strains in Japan
Emergence of the community-acquired methicillin-resistant Staphylococcus aureus USA300 clone in a Japanese child, demonstrating multiple divergent strains in Japan
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DOI：
10.1007/s10156-010-0051-y
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发表时间：
2010-08-01
影响因子：
2.2
通讯作者：
Yamamoto, Tatsuo
中科院分区：
文献类型：
--
作者：
Higuchi, Wataru;Mimura, Shigenao;Yamamoto, Tatsuo
2008年，我们从一名居住在日本埼玉县的11个月大的日本女孩身上分离出耐甲氧西林金黄色葡萄球菌（MRSA），她患有大腿下部蜂窝组织炎和败血症。 MRSA（NN47株）属于多位点序列类型（ST）8，并表现出spa363（t024）、agr1、葡萄球菌盒式染色体mec（SCCmec）IVa型和凝固酶III型。潘顿瓦伦丁杀白细胞素 (PVL) 和精氨酸分解代谢移动元件 (ACME) 呈阳性。脉冲场凝胶电泳 (PFGE) 证明 MRSA 是 USA300 克隆，它是美国主要的社区获得性 MRSA (CA-MRSA)。就 spa 类型以及 PFGE 和质粒的模式而言，菌株 NN47 与 USA300-0114 型菌株或 USA300 菌株 NN36 不同，后者先前从来自美国的访客（印度女孩）中分离出来。除了 β-内酰胺类药物（例如苯唑西林）外，菌株 NN47 对红霉素也具有耐药性。这些数据表明，USA300克隆首次出现在从未出国且未与外国人接触的日本儿童中（因此，USA300首次在日本传播），并且在日本出现了多个不同的USA300克隆株。由于 USA300 克隆具有高度传播性和毒力，因此需要对 USA300 克隆进行监测。
In 2008 we isolated methicillin-resistant Staphylococcus aureus (MRSA) from an 11-month-old Japanese girl who lived in Saitama, Japan, and suffered from cellulitis of the lower thigh and sepsis. The MRSA (strain NN47) belonged to multilocus sequence type (ST) 8 and exhibited spa363 (t024), agr1, staphylococcal cassette chromosome mec (SCCmec) type IVa, and coagulase type III. It was positive for Panton-Valentine leukocidin (PVL) and the arginine catabolic mobile element (ACME). Pulsed-field gel electrophoresis (PFGE) demonstrated that the MRSA was the USA300 clone, which is the predominant community-acquired MRSA (CA-MRSA) in the US. Strain NN47 was divergent, in terms of the spa type and patterns of PFGE and plasmids, from the USA300-0114 type strain or USA300 strain NN36, previously isolated from a visitor (Indian girl) from the US. Strain NN47 was resistant to erythromycin, in addition to beta-lactam agents (e.g., oxacillin). These data demonstrate the first emergence of the USA300 clone in Japanese children who have never been abroad and have had no contact with foreigners (and therefore, the first USA300 spread in Japan), and also emergence of multiple divergent strains of the USA300 clone in Japan. Because the USA300 clone is highly transmissible and virulent, surveillance of the USA300 clone is needed.