NIM1 overexpression in arabidopsis potentiates plant disease resistance and results in enhanced effectiveness of fungicides
NIM1 overexpression in arabidopsis potentiates plant disease resistance and results in enhanced effectiveness of fungicides
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DOI：
10.1094/mpmi.2001.14.9.1114
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发表时间：
2001-09-01
影响因子：
3.5
通讯作者：
Ryals, J
中科院分区：
文献类型：
--
作者：
Friedrich, L;Lawton, K;Ryals, J
NIM1(用于非诱导免疫，也称为NPR1)基因是拟南芥系统获得性抗性(SAR)的生物和化学激活所必需的。NIM1在野生型植物(以下简称NIM1植物或FINE)中的过表达导致了对不同病原菌不同程度的抗性。进行了实验，以解决在NIM1植物中看到的增强抗病反应的基础。在NIM1品系中观察到的抗性增加与NIM1蛋白水平的增加和PR1基因表达的快速诱导有关，PR1基因是拟南芥SAR诱导的标志。与野生型植物相比，诱导SAR所需的内源信号分子水杨酸(SA)的水平并没有显著增加。然而，在NIM1植物中，SA是增强抗性所必需的，这表明NIM1过表达的影响是植物对SA或SA依赖信号的反应更快。这一假说得到了NIM1系对SAR诱导化合物苯并(1，2，3)-噻二唑-7-甲硫基-S甲酯的高反应性的支持。此外，在NIM1植物中观察到三种杀菌剂的药效增加，这表明转基因和化学方法的结合可能导致有效和持久的疾病控制策略。
The NIM1 (for noninducible immunity, also known as NPR1) gene is required for the biological and chemical activation of systemic acquired resistance (SAR) in Arabidopsis. Overexpression of NIM1 in wild-type plants (hereafter referred to as NIM1 plants or fines) results in varying degrees of resistance to different pathogens. Experiments were performed to address the basis of the enhanced disease resistance responses seen in the NIM1 plants. The increased resistance observed in the NIM1 lines correlated with increased NIM1 protein levels and rapid induction of PR1 gene expression, a marker for SAR induction in Arabidopsis, following pathogen inoculation. Levels of salicylic acid (SA), an endogenous signaling molecule required for SAR induction, were not significantly increased compared with wild-type plants. SA was required for the enhanced resistance in NIM1 plants, however, suggesting that the effect of NIM1 overexpression is that plants are more responsive to SA or a SA-dependent signal. This hypothesis is supported by the heightened responsiveness that NIM1 lines exhibited to the SAR-inducing compound benzo(1,2,3)-thiadiazole-7-car-bothioic add S-methyl ester. Furthermore, the increased efficacy of three fungicides was observed in the NIM1 plants, suggesting that a combination of transgenic and chemical approaches may lead to effective and durable disease-control strategies.