Investigation of a tellurium-packed column for isolation of astatine-211 from irradiated bismuth targets and demonstration of a semi-automated system
Investigation of a tellurium-packed column for isolation of astatine-211 from irradiated bismuth targets and demonstration of a semi-automated system
复制标题
研究用于从辐照铋靶材中分离砹 211 的碲填充柱并演示半自动化系统
作者：
Yawen Li;D. Hamlin;M. Chyan;Taylor M. Morscheck;Maryline G. Ferrier;R. Wong;D. Wilbur
Astatine-211是一种有吸引力的放射性核素，用于血液传播疾病和微转移性疾病的靶向α治疗。有效的分离方法，可适用于强大的自动化211 At的隔离系统是高度关注的，以提高211 At的可用性。基于Bochvarova等人从辐照钍靶中分离211 At的早期研究，我们开发了一种使用碲填充柱从铋靶中分离211 At的方法。辐照铋靶的溶解是使用硝酸完成的;然而，211 At不会被捕获在该基质中的Te柱材料上。我们的方法包括缓慢添加NH 2 OH水溶液到溶解在HNO 3中的Bi靶中以转化为HCl基质。优化了NH 2 OH·HCl的量，因为（1）所用NH 2 OH·HCl的量似乎会影响苯乙基-Closo-十硼酸酯（2-）（B10）结合抗体的放射性标记产率，（2）减少NH 2 OH·HCl溶液的体积可有效缩短总分离时间。使用含有~0.96 GBq（~26 mCi）211 At的靶，已经证明了概念验证半自动化过程。获得了高的分离产率（88-95%）。通过放射性HPLC评估分离的211 At的放射化学纯度。使用ICP-MS评估211 At和含astatinated抗体中Bi和Te污染物的浓度。
Astatine-211 is an attractive radionuclide for use in targeted alpha therapy of blood-borne diseases and micrometastatic diseases. Efficient isolation methods that can be adapted to robust automated 211At isolation systems are of high interest for improving the availability of 211At. Based on the early studies of Bochvarova and co-workers involving isolation of 211At from irradiated thorium targets, we developed a method for 211At isolation from bismuth targets using tellurium-packed columns. Dissolution of irradiated bismuth targets is accomplished using HNO3; however, 211At is not captured on the Te column material in this matrix. Our method involves slow addition of aqueous NH2OH·HCl to the Bi target dissolved in HNO3 to convert to a HCl matrix. The amount of NH2OH·HCl was optimized because (1) the quantity of NH2OH·HCl used appears to affect the radiolabeling yield of phenethyl-closo-decaborate(2-) (B10)-conjugated antibodies and (2) reducing the volume of NH2OH·HCl solution can effectively shorten the overall isolation time. A proof-of-concept semi-automated process has been demonstrated using targets containing ~0.96 GBq (~26 mCi) of 211At. High isolation yields (88–95%) were obtained. Radiochemical purity of the isolated 211At was assessed by radio-HPLC. Concentrations of Bi and Te contaminants in the 211At and the astatinated antibodies were evaluated using ICP-MS.
影响因子：
4.7
作者：
Wilbur, D. Scott;Chyan, Ming-Kuan;Hawthorne, M. Frederick
通讯作者：
Hawthorne, M. Frederick