An endoderm specific GATA factor gene, dGATAe, is required for the terminal differentiation of the Drosophila endoderm
An endoderm specific GATA factor gene, dGATAe, is required for the terminal differentiation of the Drosophila endoderm
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DOI：
10.1016/j.ydbio.2004.11.021
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发表时间：
2005-02-15
影响因子：
2.7
通讯作者：
Murakami, R
中科院分区：
文献类型：
--
作者：
Okumura, T;Matsumoto, A;Murakami, R
GATA因子在原口动物和后口动物的内胚层规范中都起着至关重要的作用。在果蝇中，GATA因子基因蛇（srp）对内胚层的分化至关重要。然而，srp的表达在II期左右消失，这比中肠（一个完全的内胚层器官）的明显分化要早得多。我们已经鉴定出另一种果蝇内胚层特异性GATA因子基因，dGATAe。dGATAe在第8期内胚层中首次被检测到表达，并在整个生命周期内持续在内胚层中肠中表达。dGATAe的表达需要srp， srp的错表达导致dGATAe的异位表达。缺少dGATAe或注射dGATAe对应的双链RNA （dsRNA）的胚胎不能表达分化中肠特征的标记基因。相反，即使在没有srp活性的情况下，dGATAe的过表达也会诱导内胚层标志物的异位表达。转染dGATAe cDNA也能诱导果蝇S2细胞的内胚层标记物。这些研究提供了果蝇内胚层形成的遗传途径的概要。该途径通过母体躯干基因、末端间隙基因哈克因（hkb）以及两个GATA因子基因srp和dGATAe的顺序信号触发。(C) 2004爱思唯尔公司版权所有。
GATA factors play an essential role in endodermal specification in both protostomes and deuterostomes. In Drosophila, the GATA factor gene serpent (srp) is critical for differentiation of the endoderm. However, the expression of srp disappears around stage II, which is much earlier than overt differentiation occurs in the midgut, an entirely endodermal organ. We have identified another endoderm-specific Drosophila GATA factor gene, dGATAe. Expression of dGATAe is first detected at stage 8 in the endoderm, and its expression continues in the endodermal midgut throughout the life cycle. srp is required for expression of dGATAe, and misexpression of srp resulted in ectopic dGATAe expression. Embryos that either lacked dGATAe or were injected with double-stranded RNA (dsRNA) corresponding to dGATAe failed to express marker genes that are characteristic of differentiated midgut. Conversely, overexpression of dGATAe induced ectopic expression of endodermal markers even in the absence of srp activity. Transfection of the dGATAe cDNA also induced endodermal markers in Drosophila S2 cells. These studies provide an outline of the genetic pathway that establishes the endoderm in Drosophila. This pathway is triggered by sequential signaling through the maternal torso gene, a terminal gap gene, huckebein (hkb), and finally, two GATA factor genes, srp and dGATAe. (C) 2004 Elsevier Inc. All rights reserved.