Segmental diversity of electrogenic glucose transport characteristics in the small intestines of weaned pigs
Segmental diversity of electrogenic glucose transport characteristics in the small intestines of weaned pigs
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DOI：
10.1016/j.cbpa.2012.05.204
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发表时间：
2012-09-01
影响因子：
2.3
通讯作者：
Breves, Gerhard
中科院分区：
文献类型：
--
作者：
Herrmann, Jens;Schroeder, Bernd;Breves, Gerhard
一些物种的研究表明，肠钠离子依赖的葡萄糖协同转运体1(SGLT1)在空肠中比在回肠中更丰富。相比之下，哺乳仔猪或断奶仔猪的肠道葡萄糖摄取率的效率并不清楚地符合这种分段分布。本研究的目的是更详细地评价SGLT1介导的生长猪(Sus Scrofa)空肠和回肠葡萄糖吸收。在Ussing小室中，空肠的基础短路电流明显更正。可以证明，不同品种的回肠葡萄糖电转运明显更明显，在5 mmol.L-1葡萄糖在回肠中的表达速度是回肠的7倍，尽管Western blotting检测到空肠糖基化SGLT1的表达略高。这种表达模式与空肠对根皮苷的敏感度显著降低有关。通过对分离的刷状缘膜囊泡的葡萄糖吸收研究，发现两段膜的Na+/K+-ATPase的丰度和活性没有差异，因此，猪回肠葡萄糖转运特性的节段性差异可能是基于SGLT1活性的直接或间接调节。(C)2012 Elsevier Inc.保留所有权利。
It has been shown in several species that the intestinal Na+-dependent glucose co-transporter 1 (SGLT1) is more abundant in the jejunum than in ileum. In contrast, the efficiency of intestinal glucose uptake rates in suckling piglets or weaned pigs is not clearly fitting with this segmental distribution. The aim of this study was to evaluate SGLT1 mediated glucose absorption in the jejunum and ileum of growing pigs (Sus scrofa) in more detail. In Ussing chambers, basal short-circuit currents were significantly more positive in the jejunum. It could be demonstrated that the electrogenic ileal glucose transport was significantly more pronounced in different breeds and occurred at 5 mmol.L-1 glucose 7 times faster in the ileum, although slightly higher jejunal expression of glycosylated SGLT1 was detected by Western blotting. This expression pattern was connected to significantly lower phlorizin sensitivity in the jejunum. As the more efficient ileal glucose absorption was also observable with glucose uptake studies into isolated brush-border membrane vesicles without differences in abundance and activity of the Na+/K+-ATPase in both segments, we conclude that the segmental differences in porcine glucose transport characteristics may be based on direct or indirect modulations of SGLT1 activity. (C) 2012 Elsevier Inc. All rights reserved.