Detection of circulating colorectal cancer cells by a custom microfluid system before and after endoscopic metallic stent placement
Detection of circulating colorectal cancer cells by a custom microfluid system before and after endoscopic metallic stent placement
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DOI：
10.3892/ol.2019.11047
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发表时间：
2019-12-01
期刊：
影响因子：
2.9
通讯作者：
Koike, Kazuhiko
中科院分区：
文献类型：
--
作者：
Ishibashi, Rei;Yoshida, Shuntaro;Koike, Kazuhiko
尽管循环肿瘤细胞（CTC）的检测对于未来的个性化医疗至关重要，但尚未建立有效且灵活的方法。本研究建立了一种聚合物定制芯片，用于捕获具有高效率和灵活性的CTC。作为临床应用实例，分析了自膨式金属支架（SEMS）置入对结直肠癌和肠梗阻患者癌细胞释放到血液中的影响。这被评估为SEMS的放置可能对恶性组织造成机械损伤和物理力，增加癌细胞释放到血流中的风险。本研究使用定制的芯片检查了结肠直肠癌患者SEMS放置前、放置后24小时和放置后4天的CTC数量。结果显示，在13例接受检查的患者中，有3例在SEMS放置后24 h CTCs数量增加。然而，这种增长是暂时的。在大多数情况下，CTC的数量在支架放置后4天也减少。当前研究的CTC芯片检测到CD133阳性癌症干细胞样细胞的数量，即使在CTC总数暂时增加的患者中也没有变化。结果表明，这种定制的微流体系统可以有效和灵活地检测CTC，展示了其在癌症患者管理过程中获取信息的潜力。
Although the detection of circulating tumor cells (CTCs) should be crucial for future personalized medicine, no efficient and flexible methods have been established. The current study established a polymeric custom-made chip for capturing CTCs with a high efficiency and flexibility. As an example of clinical application, the effects of self-expandable metallic stent (SEMS) placement on the release of cancer cells into the blood of patients with colorectal cancer and bowel obstruction were analyzed. This was assessed as the placement of SEMS may cause mechanical damage and physical force to malignant tissue, increasing the risk of cancer cell release into the bloodstream. The present study examined the number of CTCs using a custom-made chip, before, at 24 h after and at 4 days after SEMS placement in patients with colorectal cancer. The results revealed that, among the 13 patients examined, the number of CTCs was increased in three cases at 24 h after SEMS placement. However, this increase was temporary. The number of CTCs also decreased at 4 days after stent placement in most cases. The CTC chip of the current study detected the number of CD133-positive cancer stem-like cells, which did not change, even in the patient whose total number of CTCs temporarily increased. The results indicated that this custom-made microfluid system can efficiently and flexibly detect CTCs, demonstrating its potential for obtaining information during the management of patients with cancer.