Production of normal young following transfer of mouse embryos obtained by in vitro fertilization between cryopreserved gametes.
Production of normal young following transfer of mouse embryos obtained by in vitro fertilization between cryopreserved gametes.
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通过冷冻保存的配子之间的体外受精获得的小鼠胚胎移植后产生正常幼仔。
DOI：
10.1530/jrf.0.0990077
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
N. Nakagata
中科院分区：
文献类型：
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作者：
N. Nakagata
将小鼠未受精卵母细胞（C57BL/6N和C3H/He）和附睾精子（DBA/2N和Jcl:ICR）分别冷冻保存在-196℃，解冻后分别用C57BL/6N卵母细胞和DBA/2N、C57BL/6N卵母细胞和Jcl:ICR、C3H/He卵母细胞和DBA/2N、C3H/He卵母细胞和Jcl:ICR进行体外受精。在假妊娠的第一天（确认阴道堵塞的当天），将体外培养到双细胞期的胚胎移植到女性受体的输卵管中。各组双细胞胚胎发育率在22 ~ 45%之间。当这些双细胞胚胎全部移植到受体后，23-35%的胚胎产生了后代。
Unfertilized oocytes (C57BL/6N and C3H/He) and epididymal spermatozoa (DBA/2N and Jcl:ICR) from mice were frozen separately and stored at -196 degrees C. After thawing, in vitro fertilization was performed using C57BL/6N oocytes and DBA/2N spermatozoa, C57BL/6N oocytes and Jcl:ICR spermatozoa, C3H/He oocytes and DBA/2N spermatozoa, and C3H/He oocytes and Jcl:ICR spermatozoa. Embryos developed to the two-cell stage by incubation in vitro were transferred to the oviducts of female recipients on the first day of pseudopregnancy (day when vaginal plug was confirmed). The rate of development to two-cell embryos in each group was in the 22-45% range. When these two-cell embryos were all transferred to recipients, offspring were produced from 23-35% of the embryos.