SENSITIVE ASSAYS FOR VIRAL ANTIBODIES IN SALIVA - AN ALTERNATIVE TO TESTS ON SERUM
SENSITIVE ASSAYS FOR VIRAL ANTIBODIES IN SALIVA - AN ALTERNATIVE TO TESTS ON SERUM
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DOI：
10.1016/s0140-6736(87)92737-1
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发表时间：
1987-07-11
期刊：
影响因子：
168.9
通讯作者：
MORTIMER, PP
中科院分区：
文献类型：
--
作者：
PARRY, JV;PERRY, KR;MORTIMER, PP
配对血清和唾液标本采用常规测定法、igg捕获放射免疫测定法（GACRIA）和酶联免疫吸附测定法（GACELISA）检测甲型肝炎病毒（HAV， 100对）、人类免疫缺陷病毒（HIV， 53对）、乙型肝炎病毒核心（HBc， 62对）和风疹病毒（30对）的抗体。在119名血清阳性受试者中，常规检测方法未能检测到病毒抗体。然而，除2例唾液标本抗hbc假阴性和1例抗风疹病毒假阴性外，GACRIA在常规检测中均检测到血清和唾液抗体阳性。血清中抗hiv和抗hbc的GACRIA反应性与唾液中无显著差异，但血清中抗hav和风疹病毒的GACRIA反应性强于唾液。GACRIA和GACELISA在唾液中的检测结果。抗体密切相关；GACELISA和GACRIA在抗hav和抗hiv方面的准确性相同。对于唾液和血清，GACRIA在检测抗hav和抗hiv方面优于iga捕获法。
Paired serum and saliva specimens were tested by conventional assays and by IgG-capture radioimmunoassays (GACRIA) and enzyme-linked immunosorbent assays (GACELISA) for antibody to hepatitis A virus (HAV, 100 pairs), human immunodeficiency virus (HIV, 53), hapatitis B virus core (HBc, 62), and rubella virus (30). Conventional assays failed to detect viral antibodies in the saliva of 93 of 119 seropositive subjects. However, GACRIA detected the antibodies in both serum and saliva of all subjects seropositive by conventional tests, except 2 saliva specimens false-negative for anti-HBc and 1 false-negative for anti-rubella-virus. For anti-HIV and anti-HBc serum and saliva GACRIA reactivities did not differ significantly, but anti-HAV and anti-rubella-virus GACRIA reactivities were stronger in serum than saliva. GACRIA and GACELISA results on saliva for the four .cntdot. antibodies correlated closely; for anti-HAV and anti-HIV GACELISA and GACRIA were equally accurate. For both saliva and serum, GACRIA was superior to an IgA-capture assay in detecting anti-HAV and anti-HIV.