Ethanol Induced Brain Lipid Changes in Mice Assessed by Mass Spectrometry.
Ethanol Induced Brain Lipid Changes in Mice Assessed by Mass Spectrometry.
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通过质谱法评估乙醇诱导的小鼠脑脂质变化。
DOI：
10.1021/acschemneuro.6b00120
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发表时间：
2016
影响因子：
5
通讯作者：
Woods,AminaS
中科院分区：
文献类型：
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作者：
Roux,Aurelie;Jackson,ShelleyN;Muller,Ludovic;Barbacci,Damon;O'Rourke,Joseph;Thanos,PanayotisK;Volkow,NoraD;Balaban,Carey;Schultz,JAlbert;Woods,AminaS
酒精滥用是一种慢性疾病，其特征是饮酒量达到干扰身心健康的水平，并导致大脑发生严重和持续的变化。脂质代谢由于其在脑中的高浓度而特别令人感兴趣。脂质是细胞膜的主要成分，参与细胞信号传导、信号转导和能量储存。在这项研究中，我们分析了长期乙醇暴露小鼠大脑的脂质组成。将幼年（JUV）和成年（ADU）小鼠置于每日限制乙醇摄入模型中52天。安乐死后，收获脑，并从脑匀浆中提取总脂质。使用高分辨率质谱分析样品，并通过多变量和单变量统计分析进行处理。在不同类别中观察到显著的脂质变化，包括鞘脂、脂肪酸、溶血磷脂酰胆碱和其他甘油磷脂。
Alcohol abuse is a chronic disease characterized by the consumption of alcohol at a level that interferes with physical and mental health and causes serious and persistent changes in the brain. Lipid metabolism is of particular interest due to its high concentration in the brain. Lipids are the main component of cell membranes, are involved in cell signaling, signal transduction, and energy storage. In this study, we analyzed lipid composition of chronically ethanol exposed mouse brains. Juvenile (JUV) and adult (ADU) mice were placed on a daily limited-access ethanol intake model for 52 days. After euthanasia, brains were harvested, and total lipids were extracted from brain homogenates. Samples were analyzed using high resolution mass spectrometry and processed by multivariate and univariate statistical analysis. Significant lipid changes were observed in different classes including sphingolipids, fatty acids, lysophosphatidylcholines, and other glycerophospholipids.