Ethanol stimulation of HIV infection of oral epithelial cells
Ethanol stimulation of HIV infection of oral epithelial cells
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DOI：
10.1097/01.qai.0000129572.13008.db
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发表时间：
2004-12-01
影响因子：
3.6
通讯作者：
Pang, S
中科院分区：
文献类型：
--
作者：
Zheng, JY;Yang, OO;Pang, S
在接受性口交或母乳喂养过程中，口腔粘膜细胞可被外源性HIV感染。口腔粘膜感染的风险取决于口腔中存在的HIV毒株的感染效率、病毒滴度和口腔环境中的防御机制。预计酒精会削弱宿主对口腔中艾滋病毒感染的防御机制。我们通过插入增强型绿色荧光蛋白基因对HIV毒株NL 4 -3进行了修饰，并使用该病毒感染从患者体内获得的口腔上皮细胞。将各种浓度的乙醇（0%-4%）加入到感染的细胞中。通过荧光显微镜或荧光激活细胞分选检测HIV感染的细胞。我们发现，乙醇显着增加艾滋病毒感染的原代口腔上皮细胞（POE）。用4%乙醇预处理少于10分钟的POE显示出对CXCR-4 HIV毒株NL 4 -3感染的敏感性高3至6倍。我们的研究还表明，HIV通过一种不依赖于gp 120的机制感染POE。我们测试了一种HIV CCR 5株，JRCSF，也发现它的感染效率被酒精刺激。我们的研究结果表明，在细胞培养条件下，市售的酒精浓度范围能够刺激HIV在POE中的感染效率。
Oral mucosal cells can be infected by exogenous HIV during receptive oral sex or breast-feeding. The risk of oral mucosal infection depends on the infection efficiency of the HIV strains present in the oral cavity, the viral titers, and the defense mechanisms in the oral cavity environment. It is expected that alcohol can weaken the host defense mechanism against HIV infection in the oral cavity. We modified an HIV strain, NL4-3, by inserting the enhanced green fluorescent protein gene and used this virus to infect oral epithelial cells obtained from patients. Various concentrations of ethanol (0%-4%) were added to the infected cells. HIV-infected cells were detected by fluorescent microscopy or fluorescence-activated cell sorting. We found that ethanol significantly increases HIV infection of primary oral epithelial cells (POEs). POEs pretreated with 4% ethanol for less than 10 minutes demonstrated 3- to 6-fold higher susceptibility to infection by the CXCR-4 HIV strain NL4-3. Our studies also demonstrated that HIV infects POEs through a gp120-independent mechanism. We tested an HIV CCR5 strain, JRCSF, and also found its infection efficiency to be stimulated by alcohol. Our results indicate that in cell culture conditions, the ranges of concentrations of alcohol that are commercially available are able to stimulate the infection efficiency of HIV in POEs.