NEW MEDIUM FOR THE PRODUCTION OF CHOLERA-TOXIN BY VIBRIO-CHOLERAE 01 BIOTYPE EL-TOR
NEW MEDIUM FOR THE PRODUCTION OF CHOLERA-TOXIN BY VIBRIO-CHOLERAE 01 BIOTYPE EL-TOR
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DOI：
10.1128/jcm.22.3.405-408.1985
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发表时间：
1985-01-01
影响因子：
9.4
通讯作者：
YAMAMOTO, K
中科院分区：
文献类型：
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作者：
IWANAGA, M;YAMAMOTO, K
开发了一种刺激霍乱弧菌O1 El Tor（El Tor vibrios）体外产生霍乱毒素的新培养基。培养基含有0.5% NaCl、0.3% NaHCO3、0.4%酵母提取物和1.5%细菌蛋白胨。将El Tor弧菌在37 ℃下在固定试管中培养。C下保温20h。通过反向被动乳胶凝集法测定培养物上清液中的霍乱毒素。在此培养基中生长的大多数弧菌产生的毒素比传统的syncase培养基高10 - 20倍。在新培养基（AKI培养基）中，培养结束时活弧菌的数量约为108/ml，在syncase培养基中约为1010/ml。因此，新培养基中的每一个微生物产生的毒素应该是syncase培养基中的1,000倍。在新培养基中，碳酸氢钠是影响El Tor弧菌产毒的最重要因素。这种新培养基由于其霍乱毒素产量高和技术简单而被推荐。
A new medium that stimulates in vitro production of cholera toxin by V. cholerae O1 El Tor (El Tor vibrios) was developed. The medium contains 0.5% NaCl, 0.3% NaHCO3, 0.4% yeast extract, and 1.5% Bacto-Peptone. El Tor vibrios were cultured in a stationary test tube at 37.degree. C for 20 h. The culture supernatant was assayed for cholera toxin by a reversed passived latex agglutination method. Most vibrios grown in this medium produced 10-20 times more toxin than in traditional syncase medium. The number of live vibrios at the end of culture was about 108/ml in the new medium (AKI medium) and about 1010/ml in the syncase medium. As a result, each individual organism in the new medium should have produced as much as 1,000 times more toxin than in syncase medium. Sodium bicarbonate was found to be the most important factor in toxin production by El Tor vibrios in the new medium. This new medium is recommended because of its high yield of cholera toxin and its technical simplicity.