In vitro and in vivo activity of melaleuca alternifolia mixed with tissue conditioner on Candida albicans
In vitro and in vivo activity of melaleuca alternifolia mixed with tissue conditioner on Candida albicans
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DOI：
10.1016/j.tripleo.2007.08.025
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发表时间：
2008-03-01
期刊：
影响因子：
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通讯作者：
Mondaca, Maria A.
中科院分区：
文献类型：
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作者：
Catalan, Alfonso;Pacheco, Juan G.;Mondaca, Maria A.
Objective.本研究的目的是鉴定与不同组织调理剂混合的互叶白千层油对白色念珠菌菌株的体外和体内活性。采用微生物学试验对义齿性口炎患者进行白色念珠菌的分离。测定了互叶白千层在直接施用和与组织调理剂（Fitt、Lynal、Coe-Comfort）混合时对白色念珠菌的体外抗真菌活性。对27例义齿性口炎患者进行为期12天的治疗，分别采用互叶白千层与Coe-Comfort（n = 9）、制霉菌素与Coe-Comfort（n = 9）和Coe-Comfort（对照组，n = 9）。在体外研究中，Coe-Comfort或Fitt护发素与1 mL 20%（体积/体积）互叶白千层油混合后，对白色念珠菌表现出完全抑制作用。用M.与Coe-Comfort处理组相比，Alternifolia与Coe-Comfort混合处理组的腭部炎症显著减少（P = .001）。此外，C.用M.与Coe-Comfort相比，Alternifolia与Coe-Comfort混合使用的效果更好（P = .000004）。M. Alternifolia油与Coe-Comfort组织调理剂混合可有效治疗义齿性口炎。
Objective. The aim of this study was to identify in vitro and in vivo activity of Melaleuca alternifolia oil mixed with different tissue conditioners on the Candida albicans strain.Study design. Microbiological tests were used to isolate Candida albicans from patients with denture stomatitis. The in vitro antifungal activity of Melaleuca alternifolia against Candida albicans was determined when it was applied directly and when it was mixed with tissue conditioners (Fitt, Lynal, Coe-Comfort). The responses of 27 denture stomatitis patients treated with Melaleuca alternifolia mixed with Coe-Comfort (n = 9), Nystatin mixed with Coe-Comfort (n = 9), and Coe-Comfort (Control Group, n = 9), were evaluated over a period of 12 days.Results. In the in vitro study, Coe-Comfort or Fitt conditioners mixed with 1 mL, 20% (vol/ vol) of Melaleuca alternifolia oil exhibited a total inhibition of Candida albicans. Patients treated with M. alternifolia mixed with Coe-Comfort showed a significant decrease in palatal inflammation compared with those treated with Coe Comfort (P = .001). In addition, a significant inhibition of C. albicans growth was observed with M. alternifolia mixed with Coe-Comfort compared with only Coe-Comfort (P = .000004).Conclusion. M. alternifolia oil mixed with Coe-Comfort tissue conditioner is effective in treating denture stomatitis.