DETECTION OF HIGHLY POLYMORPHIC MICROSATELLITE LOCI IN A SPECIES WITH LITTLE ALLOZYME POLYMORPHISM
DETECTION OF HIGHLY POLYMORPHIC MICROSATELLITE LOCI IN A SPECIES WITH LITTLE ALLOZYME POLYMORPHISM
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DOI：
10.1111/j.1365-294x.1993.tb00102.x
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发表时间：
1993-06-01
影响因子：
4.9
通讯作者：
QUELLER, DC
中科院分区：
文献类型：
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作者：
HUGHES, CR;QUELLER, DC
微卫星是含有短序列基序串联重复序列的DNA区域，广泛存在于所有真核生物基因组中，是人类和其他哺乳动物高度多态遗传标记的丰富来源。这些基因座特别适合于群体研究，因为它们可以相对容易地通过聚合酶链式反应(PCR)扩增每个基因座并随后进行电泳法分离等位基因来得分。本文详细介绍了一种在任何物种中寻找这些基因座的方法。这种方法表明，在群居黄蜂中，三核苷酸微卫星座位丰富且高度多态，而等位酶电泳法显示的多态很少。前6个座位均为多态，平均杂合度为0.62，33个等位基因的平均杂合度为0.035。我们建议，这种方法可以用来检测其他方法失败的变异，使其成为种群和保护遗传学家的理想工具，他们必须处理缺乏其他类型遗传变异性的种群。
Microsatellite loci are regions of DNA containing tandem repeats of a short sequence motif; they occur abundantly in all eukaryotic genomes and have been shown to be a rich source of highly polymorphic genetic markers in humans and other mammals. These loci are particularly suitable for population studies because they can be relatively easily scored using a combination of polymerase chain reaction (PCR) amplification of each locus followed by electrophoresis to separate alleles. This paper details a method for finding these loci in any species. This method demonstrates that trinucleotide microsatellite loci are abundant and highly polymorphic in the social wasp Polistes annularis, whereas allozyme electrophoresis reveals very little polymorphism. The first six loci examined were all polymorphic with a mean observed heterozygosity of 0.62; in comparison average heterozygosity of 33 allozymes was 0.035. We suggest that this method can be used to detect variation where other methods have failed, making it an ideal tool for population and conservation geneticists who must deal with populations lacking other types of genetic variability.