Listeria monocytogenes varies among strains to maintain intracellular pH homeostasis under stresses by different acids as analyzed by a high-throughput microplate-based fluorometry.
Listeria monocytogenes varies among strains to maintain intracellular pH homeostasis under stresses by different acids as analyzed by a high-throughput microplate-based fluorometry.
复制标题
通过基于高通量微孔板的荧光测定法分析，单核细胞增生李斯特菌在不同酸的胁迫下维持细胞内 pH 稳态的菌株之间存在差异
DOI：
10.3389/fmicb.2015.00015
复制
发表时间：
2015
影响因子：
5.2
通讯作者：
Song H
中科院分区：
文献类型：
--
作者：
Cheng C;Yang Y;Dong Z;Wang X;Fang C;Yang M;Sun J;Xiao L;Fang W;Song H
单核细胞增生李斯特菌是一种食源性致病菌，在酸性环境中具有维持细胞内pH（pHi）稳态的能力，但其潜在机制仍不清楚。在这里，我们报告了一个简单的基于微孔板的荧光方法来确定pHi的pHi与荧光染料羧基荧光素二乙酸酯N-琥珀酰亚胺酯预标记，并进行酸应力的细胞。我们发现L.单核细胞增生对有机酸和无机酸的反应在菌株之间不同，以维持pHi稳态。L的容量。在乙酸和乳酸的存在下，单核细胞增多症维持细胞外pH 4.5（pHex）的pHi的能力受到损害，但不受盐酸和柠檬酸的影响。在一定的pH条件下，有机酸的抑制效果比盐酸强。此外，强毒菌株L.单核细胞增生菌EGDe、850658和10403 S比在维持pHi稳态方面表现出缺陷的无毒M7更耐酸性胁迫。sigB，从10403 S应激反应的替代西格玛因子的缺失，显着改变细胞内的pH值的稳态，并显示出显着的生长和生存缺陷在酸性条件下。因此，这项工作提供了新的见解细菌的生存机制，以酸性胁迫。
Listeria monocytogenes, a food-borne pathogen, has the capacity to maintain intracellular pH (pHi) homeostasis in acidic environments, but the underlying mechanisms remain elusive. Here, we report a simple microplate-based fluorescent method to determine pHi of listerial cells that were prelabeled with the fluorescent dye carboxyfluorescein diacetate N-succinimidyl ester and subjected to acid stress. We found that L. monocytogenes responds differently among strains toward organic and inorganic acids to maintain pHi homeostasis. The capacity of L. monocytogenes to maintain pHi at extracellular pH 4.5 (pHex) was compromised in the presence of acetic acid and lactic acid, but not by hydrochloric acid and citric acid. Organic acids exhibited more inhibitory effects than hydrochloric acid at certain pH conditions. Furthermore, the virulent stains L. monocytogenes EGDe, 850658 and 10403S was more resistant to acidic stress than the avirulent M7 which showed a defect in maintaining pHi homeostasis. Deletion of sigB, a stress-responsive alternative sigma factor from 10403S, markedly altered intracellular pHi homeostasis, and showed a significant growth and survival defect under acidic conditions. Thus, this work provides new insights into bacterial survival mechanism to acidic stresses.
登录
查看更多内容
影响因子：
6.4
作者：
Palmer ME;Chaturongakul S;Wiedmann M;Boor KJ
通讯作者：
Boor KJ
影响因子：
5.4
作者：
Le Marc, Y;Huchet, V;Thuault, D
通讯作者：
Thuault, D
影响因子：
3.9
作者：
Giulitti, Stefano;Cinquemani, Claudio;Spilimbergo, Sara
通讯作者：
Spilimbergo, Sara
影响因子：
2.8
作者：
Lues, Jan Frederick Rykers;Theron, Maria Magdalena
通讯作者：
Theron, Maria Magdalena
非致病性单核细胞增生李斯特氏菌血清型 4a 菌株 M7 的基因组序列
影响因子：
3.2
作者：
Chen, Jianshun;Xia, Ye;Fang, Weihuan
通讯作者：
Fang, Weihuan