Parameters influencing measurement of the Gag antigen-specific T-proliferative response to HIV type 1 infection.
Parameters influencing measurement of the Gag antigen-specific T-proliferative response to HIV type 1 infection.
复制标题
影响对 HIV 1 型感染的 Gag 抗原特异性 T 增殖反应测量的参数。
DOI：
10.1089/088922200309359
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Moore,JP
中科院分区：
文献类型：
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作者：
Schiller,DS;Binley,JM;Roux,KH;Adamson,CS;Jones,IM;Krausslich,HG;Hurley,A;Markowitz,M;Moore,JP
我们已经分析了可能影响体外定量检测T细胞增殖反应的因素，该反应是一种常见的且越来越多地用于辅助性T细胞的临床测量。 在HIV-1感染的背景下发挥作用。我们比较了新鲜制备的和先前冷冻的PBMC样品中T细胞增殖的速率和程度，并得出结论，冷冻细胞可以 成功使用;我们已经评估了PBMC培养物中任何HIV-1复制的抑制是否会影响T细胞增殖的程度;我们已经研究了哪些形式的Gag抗原是T细胞增殖的抑制剂。 最有效地诱导T细胞增殖。从后面的实验中，我们得出结论，Gag蛋白包括p17，可能还有p7，侧接中心p24衣壳蛋白的序列， 是比仅包含p24序列的蛋白质更好的刺激物。
We have analyzed factors that might influence thein vitroquantitation of the T-proliferative response to HIV-1 Gag antigens, a common and increasingly used clinical measurement of helper T cell function in the context of HIV-1 infection. We have compared the rate and extent of T cell proliferation in freshly prepared and previously frozen PBMC samples, and have concluded that frozen cells can be used successfully; we have assessed whether the suppression of any HIV-1 replication in the PBMC cultures affects the extent of T cell proliferation; we have studied which forms of the Gag antigens are the most efficient at inducing T cell proliferation. From the latter experiments, we conclude that Gag proteins that include p17, and perhaps also p7, sequences flanking the central p24 capsid protein, are better stimulants than proteins that comprise only p24 sequences.