Three QTL regions for restoration of Owen CMS in sugar beet
Three QTL regions for restoration of Owen CMS in sugar beet
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DOI：
10.2135/cropsci2002.0540
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发表时间：
2002-03-01
期刊：
影响因子：
2.3
通讯作者：
Nilsson, NO
中科院分区：
文献类型：
--
作者：
Hjerdin-Panagopoulos, A;Kraft, T;Nilsson, NO
通过分析恢复细胞质雄性不育植物育性的三个群体的基因，确定了甜菜（Beta vulgaris L.ssp. vulgaris）中用于恢复 Owen CMS（细胞质雄性不育）的三个定量基因座（QTL）区域。分别通过 51 个和 42 个多态性和均匀分布的限制性片段长度多态性 (RFLP) 标记对两个大群体 A 和 13 进行了研究。使用六个 RFLP 标记对较小的群体 C 进行了分析。群体 A 中的复合区间作图检测到连锁群 IV 上的强信号，这解释了 79% 的表型变异。更详细的分析揭示了该染色体上存在两个连锁的QTL。在群体 B 中，一个 QTL 解释了连锁群 III 一端检测到的 72% 的表型变异。群体 C 的单标记分析导致连锁群 IV 上的标记出现显着信号，解释了 57% 的表型变异。在每个或两个大群体中，在 QTL 区域中也检测到了似乎是次要等位基因。
Three quantitative trati loci (QTL) regions for the restoration of Owen CMS (cytoplasmic male sterility) in sugar beet (Beta vulgaris L.ssp. vulgaris) were identified through analysis of three populations for genes restoring the fertility of cytoplasmic male sterile plants. Two large populations, A and 13, were studied by means of 51 and 42 polymorphic and evenly distributed restriction fragment length polymorphism (RFLP) markers, respectively. A smaller population, C, was analyzed by use of six RFLP markers. Composite interval mapping in Population A detected a strong signal on linkage group IV that explained 79% of the phenotypic variation. More detailed analysis revealed the presence of two linked QTL on this chromosome. In Population B, a QTL that explained 72% of the phenotypic variation as detected at one end of linkage group III. Single-marker analysis of Population C resulted in significant signals for markers on linkage group IV, explaining 57% of the phenotypic variation. In each or the two large populations, hat appeared to be minor alleles were also detected in the QTL regions.