An E3 Ubiquitin Ligase-BAG Protein Module Controls Plant Innate Immunity and Broad-Spectrum Disease Resistance
An E3 Ubiquitin Ligase-BAG Protein Module Controls Plant Innate Immunity and Broad-Spectrum Disease Resistance
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E3 泛素连接酶-BAG 蛋白模块控制植物先天免疫和广谱抗病性
DOI：
10.1016/j.chom.2016.10.023
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发表时间：
2016-12-14
影响因子：
30.3
通讯作者：
He, Zuhua
中科院分区：
文献类型：
--
作者：
You, Quanyuan;Zhai, Keran;He, Zuhua
植物的程序性细胞死亡（PCD）和免疫受到严格控制，以促进抗微生物防御，同时防止自身免疫。然而，促成这种免疫稳态的机制尚不清楚。在这里，我们分离了一个水稻突变体ebr1（增强的疫病和稻瘟病抗性1），它表现出增强的广谱细菌和真菌疾病抗性，但表现出自发PCD、自身免疫和生长发育迟缓。EBR1编码与OsBAG4相互作用的E3泛素连接酶，OsBAG4属于BAG （bcl -2相关的无氧基因）家族，在哺乳动物的细胞死亡、生长停滞和免疫反应中起作用。EBR1直接靶向OsBAG4进行泛素化介导的降解。水稻中OsBAG4水平的升高是触发PCD和增强抗病能力的必要和充分条件，最有可能是通过激活病原体相关的分子模式触发免疫（PTI）。总之，我们的研究表明，E3-BAG模块协调了植物的先天免疫稳态，并协调了防御和生长之间的权衡。
Programmed cell death (PCD) and immunity in plants are tightly controlled to promote antimicrobial defense while preventing autoimmunity. However, the mechanisms contributing to this immune homeostasis are poorly understood. Here, we isolated a rice mutant ebr1 (enhanced blight and blast resistance 1) that shows enhanced broad-spectrum bacterial and fungal disease resistance, but displays spontaneous PCD, autoimmunity, and stunted growth. EBR1 encodes an E3 ubiquitin ligase that interacts with OsBAG4, which belongs to the BAG (Bcl-2-associated athanogene) family that functions in cell death, growth arrest, and immune responses in mammals. EBR1 directly targets OsBAG4 for ubiquitination-mediated degradation. Elevated levels of OsBAG4 in rice are necessary and sufficient to trigger PCD and enhanced disease resistance to pathogenic infection, most likely by activating pathogen-associated molecular patterns-triggered immunity (PTI). Together, our study suggests that an E3-BAG module orchestrates innate immune homeostasis and coordinates the trade-off between defense and growth in plants.