MORPHOGENESIS AND ADHESION OF AUREOBASIDIUM-PULLULANS
MORPHOGENESIS AND ADHESION OF AUREOBASIDIUM-PULLULANS
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DOI：
10.1139/m94-002
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发表时间：
1994-01-01
影响因子：
2.8
通讯作者：
NORDHEIM, EV
中科院分区：
文献类型：
--
作者：
ANDREWS, JH;HARRIS, RF;NORDHEIM, EV
两株二型真菌出芽短梗霉生长在液体和固体培养基上不同的碳和氮含量，并在叶片表面。菌丝在所有系统中观察到，但包括一个非常低的比例（通常低于定量检测）的总生物量。在液体培养基中，仅在培养瓶壁上的冲洗区发现稀疏的菌丝。酵母相生长（芽生孢子）发生在营养平衡的pH缓冲培养基中，或连续碳限制（补料分批培养），或碳耗尽（分批培养）。芽生孢子暴露于有限的氮，但足够的有机碳的条件下，或酸化介质，转化为肿胀的细胞和厚垣孢子。后者形态型积累碳内部的脂质颗粒，然后外部的荚膜和可溶性胞外多糖。它们是有凝聚力的，并且比芽生孢子更强烈地粘附在覆盖琼脂培养基的纤维素膜上或叶子上。普鲁兰酶处理减少了胶囊，凝聚力和粘附力。在芽生孢子中加入可溶性胞外多糖可增强芽生孢子对叶片的粘附。结论：胞外多糖在A.普鲁兰多糖。
Two strains of the dimorphic fungus Aureobasidium pullulans were grown in liquid and on solid media varying in carbon and nitrogen content, and on leaf surfaces. Hyphae were observed in all systems but comprised a very low proportion (often below quantitative detection) of the total biomass. In liquid media, hyphae were found sparsely and only in the wash-zone on walls of the culture flasks. Yeast phase growth (blastospores) occurred in pH-buffered media that were nutrient balanced, or continuously carbon-limited (fed-batch culture), or carbon-exhausted (batch culture). Blastospores exposed to conditions with limited nitrogen but sufficient organic carbon, or to acidified media, converted to swollen cells and chlamydospores. The latter morphotypes accumulated carbon internally as lipid granules, and then externally as capsular and soluble extracellular polysaccharide. They were cohesive and also adhered more strongly to cellulose membranes overlying agar media or to leaves than did blastospores. Pullulanase treatment diminished the capsules, cohesion, and adhesion. Addition of soluble extracellular polysaccharide to blastospores enhanced their adhesion to leaves. We conclude that extracellular polysaccharide can play a role in adhesion of A. pullulans.