Immune parameters in the humoral fluids of amphioxus Branchiostoma belcheri challenged with Vibrio alginolyticus.
Immune parameters in the humoral fluids of amphioxus Branchiostoma belcheri challenged with Vibrio alginolyticus.
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DOI：
10.1016/j.fsi.2009.09.017
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发表时间：
2010
影响因子：
4.7
通讯作者：
Qiuxiang Pang;Shicui Zhang;Bosheng Zhao
中科院分区：
文献类型：
--
作者：
Qiuxiang Pang;Shicui Zhang;Bosheng Zhao
文昌鱼体液免疫的研究较少。本研究测定了文昌鱼感染溶藻弧菌前后体液中溶菌酶、抗菌活性、微生物凝集素和血凝素的变化。经溶藻弧菌攻击后，其溶菌酶活性、对大肠杆菌和溶藻弧菌的生长抑制活性、对溶壁微球菌、枯草芽孢杆菌和金黄色葡萄球菌的凝集活性均显著增强，对兔和人体液中A型和O型红细胞的血凝活性均提前增强。与此相反，对哈维氏弧菌和E.溶藻弧菌攻击后，体液中的大肠杆菌凝集活性降低，而对人B红细胞的凝集活性随后增加。
The knowledge concerning the humoral immunity is scarce in amphioxus Branchiostoma belcheri. This study measured the humoral parameters including lysozyme, antimicrobial activity, microbial agglutinin and haemagglutinin in amphioxus humoral fluids before and after Vibrio alginolyticus challenge. After challenged with V. alginolyticus, the lysozyme activity, growth inhibiting activities against Escherichia coli and V. alginolyticus and microbial agglutinating activities against Micrococcus lysodeikticus, Bacillus subtilis and Staphylococcus aureus were all increased significantly and haemagglutinating activities against rabbit and human A and O erythrocytes in the humoral fluids were all increased earlier. In contrast, the agglutinating activities against Vibrio harvey and E. coli in the humoral fluids were reduced in response to V. alginolyticus challenge and the haemagglutinating activity against human B erythrocytes increased later.