SOX2 expression levels distinguish between neural progenitor populations of the developing dorsal telencephalon
SOX2 expression levels distinguish between neural progenitor populations of the developing dorsal telencephalon
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DOI：
10.1016/j.ydbio.2011.01.015
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发表时间：
2011-04-01
影响因子：
2.7
通讯作者：
Pevny, Larysa H.
中科院分区：
文献类型：
--
作者：
Hutton, Scott R.;Pevny, Larysa H.
HMG-Box 转录因子 SOX2 在整个发育中和成年中枢神经系统的神经祖细胞群中表达，并且是维持其祖细胞身份所必需的。然而，目前尚不清楚 SOX2 水平是否在所有神经祖细胞群中一致表达。在发育中的背侧端脑中，两个不同的神经祖细胞群，即放射状胶质细胞和中间祖细胞，负责产生成人新皮质中发现的大部分兴奋性神经元。在这里，我们使用细胞和分子分析证明，SOX2 在放射状胶质细胞和中间祖细胞群体之间存在差异表达。此外，利用 SOX2(EGFP) 小鼠系，我们表明这种差异表达可用于前瞻性地分离不同的、存活的放射状胶质细胞和中间细胞群体，以进行体外分析。鉴于目前可用于神经祖细胞的细胞表面标记物有限，这为前瞻性地识别和分离中枢神经系统中不同类别的神经祖细胞提供了宝贵的工具。 (C) 2011 Elsevier Inc. 保留所有权利。
The HMG-Box transcription factor SOX2 is expressed in neural progenitor populations throughout the developing and adult central nervous system and is necessary to maintain their progenitor identity. However, it is unclear whether SOX2 levels are uniformly expressed across all neural progenitor populations. In the developing dorsal telencephalon, two distinct populations of neural progenitors, radial glia and intermediate progenitor cells, are responsible for generating a majority of excitatory neurons found in the adult neocortex. Here we demonstrate, using both cellular and molecular analyses, that SOX2 is differentially expressed between radial glial and intermediate progenitor populations. Moreover, utilizing a SOX2(EGFP) mouse line, we show that this differential expression can be used to prospectively isolate distinct, viable populations of radial glia and intermediate cells for in vitro analysis. Given the limited repertoire of cell-surface markers currently available for neural progenitor cells, this provides an invaluable tool for prospectively identifying and isolating distinct classes of neural progenitor cells from the central nervous system. (C) 2011 Elsevier Inc. All rights reserved.