Analysis of Cell Shape and Cell Migration of Drosophila Macrophages In Vivo.
Analysis of Cell Shape and Cell Migration of Drosophila Macrophages In Vivo.
复制标题
果蝇巨噬细胞体内细胞形状和细胞迁移分析
DOI:
10.1007/978-1-4939-7701-7_17
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发表时间:
2018
影响因子:
--
通讯作者:
Bogdan S
中科院分区:
文献类型:
--
作者:
Rüder M;Nagel BM;Bogdan S
The most abundant immune cells inDrosophilaare macrophage-like plasmatocytes that fulfill central roles in morphogenesis, immune and tissue damage response. The various genetic tools available inDrosophilatogether with high-resolution and live-imaging microscopy techniques makeDrosophilamacrophages an excellent model system that combines many advantages of cultured cells with in vivo genetics. Here, we describe the isolation and staining of macrophages from larvae for ex vivo structured illumination microscopy (SIM), the preparation of white prepupae for in vivo 2D random cell migration analysis, and the preparation of pupae (18 h after puparium formation, APF) for in vivo 3D directed cell migration analysis upon wounding using spinning disk microscopy.
DOI:
10.1083/jcb.200109057
发表时间:
2002-02-18
期刊:
The Journal of cell biology
影响因子:
--
作者:
Zallen JA;Cohen Y;Hudson AM;Cooley L;Wieschaus E;Schejter ED
通讯作者:
Schejter ED
影响因子:
7.8
作者:
Jani, Klodiana;Schoeck, Frieder
通讯作者:
Schoeck, Frieder
影响因子:
4
作者:
Brinkmann, Klaus;Winterhoff, Moritz;Bogdan, Sven
通讯作者:
Bogdan, Sven