Analysis of Cell Shape and Cell Migration of Drosophila Macrophages In Vivo.

Analysis of Cell Shape and Cell Migration of Drosophila Macrophages In Vivo.
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果蝇巨噬细胞体内细胞形状和细胞迁移分析

DOI:
10.1007/978-1-4939-7701-7_17
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发表时间:
2018
影响因子:
--
通讯作者:
Bogdan S
Bogdan S
中科院分区:
--
文献类型:
--
作者:
Rüder M;Nagel BM;Bogdan S

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果蝇体内最丰富的免疫细胞是巨噬细胞样浆细胞,它们在形态发生、免疫和组织损伤反应中起着重要作用。各种遗传工具可在果蝇与高分辨率和活成像显微镜技术使果蝇macrophages一个很好的模型系统,结合了许多优点的培养细胞与体内遗传学。在这里,我们描述了分离和染色的巨噬细胞从幼虫离体结构照明显微镜(SIM),制备白色prepuphilium在体内2D随机细胞迁移分析,和制备puphilium(18小时后,puparium形成,APF)在体内3D定向细胞迁移分析创伤后使用旋转圆盘显微镜。
The most abundant immune cells inDrosophilaare macrophage-like plasmatocytes that fulfill central roles in morphogenesis, immune and tissue damage response. The various genetic tools available inDrosophilatogether with high-resolution and live-imaging microscopy techniques makeDrosophilamacrophages an excellent model system that combines many advantages of cultured cells with in vivo genetics. Here, we describe the isolation and staining of macrophages from larvae for ex vivo structured illumination microscopy (SIM), the preparation of white prepupae for in vivo 2D random cell migration analysis, and the preparation of pupae (18 h after puparium formation, APF) for in vivo 3D directed cell migration analysis upon wounding using spinning disk microscopy.
DOI: 10.1083/jcb.200109057
发表时间: 2002-02-18
期刊: The Journal of cell biology
影响因子: --
作者:
Zallen JA;Cohen Y;Hudson AM;Cooley L;Wieschaus E;Schejter ED
通讯作者: Schejter ED
ZASP是功能整合素粘附位点组装所必需的。
DOI: 10.1083/jcb.200707045
发表时间: 2007-12-31
影响因子: 7.8
作者:
Jani, Klodiana;Schoeck, Frieder
通讯作者: Schoeck, Frieder
DOI: 10.1242/jcs.179325
发表时间: 2016-02-01
影响因子: 4
作者:
Brinkmann, Klaus;Winterhoff, Moritz;Bogdan, Sven
通讯作者: Bogdan, Sven