Oil production by oleaginous yeasts using the hydrolysate from pretreatment of wheat straw with dilute sulfuric acid
Oil production by oleaginous yeasts using the hydrolysate from pretreatment of wheat straw with dilute sulfuric acid
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DOI：
10.1016/j.biortech.2011.02.081
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发表时间：
2011-05-01
影响因子：
11.4
通讯作者：
Chen, Shulin
中科院分区：
文献类型：
--
作者：
Yu, Xiaochen;Zheng, Yubin;Chen, Shulin
探讨了利用麦草稀酸预处理水解液生产微生物油脂的方法。得到的水解物由戊糖(24.3g/L)和己糖(4.9g/L)组成，同时还有一些其他降解产物，如醋酸、糠醛和羟甲基糠醛。以该酶解液为底物，对5株产油酵母菌：隐球酵母、粘红酵母、圆环红孢子菌、脂肪酵母菌和解脂亚罗维菌进行了研究。结果表明，这些菌株均能利用脱毒后的水解液生产油脂，除圆环菌外，未脱毒的水解液也可用于所有酵母菌株的生长。在脱毒(4.2g/L)和未脱毒(5.8g/L)的水解液中，曲纹藻的脂肪含量均最高。对曲线虫的抑制作用研究表明，当浓度为3g/L时，呋喃西林对细胞生长和脂肪含量的抑制作用不明显，当浓度为1g/L时，细胞生长和脂肪含量的抑制率分别为72.0%和62.0%。我们的工作表明，脂肪生产是利用木质纤维材料前处理过程中获得的半纤维素糖的一种有前途的替代方案。(C)2011爱思唯尔有限公司。保留所有权利。
This paper explores the use of the hydrolysate from the dilute sulfuric acid pretreatment of wheat straw for microbial oil production. The resulting hydrolysate was composed of pentoses (24.3 g/L) and hexoses (4.9 g/L), along with some other degradation products, such as acetic acid, furfural, and hydroxymethylfurfural (HMF). Five oleaginous yeast strains, Cryptococcus curvatus, Rhodotorula glutinis, Rhodosporidium toruloides, Lipomyces starkeyi, and Yarrowia lipolytica, were evaluated by using this hydrolysate as substrates. The results showed that all of these strains could use the detoxified hydrolysate to produce lipids while except R. toruloides non-detoxified hydrolysate could also be used for the growth of all of the selective yeast strains. C curvatus showed the highest lipid concentrations in medium on both the detoxified (4.2 g/L) and non-detoxified (5.8 g/L) hydrolysates. And the inhibitory effect studies on C. curvatus indicated HMF had insignificant impacts at a concentration of up to 3 g/L while furfural inhibited cell growth and lipid content by 72.0% and 62.0% at 1 g/L, respectively. Our work demonstrates that lipid production is a promising alternative to utilize hemicellulosic sugars obtained during pretreatment of lignocellulosic materials. (C) 2011 Elsevier Ltd. All rights reserved.