Comprehensive Analysis of Cucumber Gibberellin Oxidase Family Genes and Functional Characterization of CsGA20ox1 in Root Development in Arabidopsis.
Comprehensive Analysis of Cucumber Gibberellin Oxidase Family Genes and Functional Characterization of CsGA20ox1 in Root Development in Arabidopsis.
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黄瓜赤霉素氧化酶家族基因综合分析及CsGA20ox1在拟南芥根系发育中的功能表征
DOI：
10.3390/ijms19103135
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发表时间：
2018-10-12
影响因子：
5.6
通讯作者：
Ren Z
中科院分区：
文献类型：
--
作者：
Sun H;Pang B;Yan J;Wang T;Wang L;Chen C;Li Q;Ren Z
黄瓜（Cucumis sativus L.）黄瓜是世界上重要的蔬菜作物，赤霉素（GAs）在黄瓜的发育和生长过程中起着重要的调节作用。GA氧化酶（GAoxs）由不同的基因亚家族编码，通过催化生物合成途径中的后续步骤在调节生物活性GA水平方面特别重要。虽然GAox是GA合成途径中的关键酶，但目前对黄瓜GAox基因的进化关系、表达谱和生物学功能的研究还很少。在本研究中，我们在黄瓜基因组中鉴定了17个GAox基因，并根据系统发育树、基因结构和保守基序将它们分为5个亚科。同线性分析表明，串联重复或片段重复事件在黄瓜GA 2 ox、GA 3 ox和GA 7 ox基因家族的扩展中起次要作用。比较同线分析结合系统发育分析提供了深入了解CsGAox基因的系统发育关系，并建议蛋白质同源性CsGAox更接近AtGAox比OsGAox。此外，预测了可能直接结合CsGAox基因启动子的候选转录因子BBR/BPC（BARLEY B RECOMBINANT/BASIC PENTACYSTEINE）和GRAS（赤霉素不敏感、GAI的回复和SCARECROW）。来自转录组数据的表达谱表明，一些CsGAox基因，特别是CsGA 20 ox 1，在幼苗根中高表达，并在GA 3处理下下调。CsGA 20 ox 1基因在拟南芥中的过量表达显著增加了主根长度和侧根数量。综上所述，对CsGAox的全面分析将为理解CsGAox家族的进化和功能提供基础。
Cucumber (Cucumis sativus L.) is an important vegetable crop worldwide and gibberellins (GAs) play important roles in the regulation of cucumber developmental and growth processes. GA oxidases (GAoxs), which are encoded by different gene subfamilies, are particularly important in regulating bioactive GA levels by catalyzing the later steps in the biosynthetic pathway. Although GAoxs are critical enzymes in GA synthesis pathway, little is known about GAox genes in cucumber, in particular about their evolutionary relationships, expression profiles and biological function. In this study, we identified 17 GAox genes in cucumber genome and classified them into five subfamilies based on a phylogenetic tree, gene structures, and conserved motifs. Synteny analysis indicated that the tandem duplication or segmental duplication events played a minor role in the expansion of cucumber GA2ox, GA3ox and GA7ox gene families. Comparative syntenic analysis combined with phylogenetic analysis provided deep insight into the phylogenetic relationships of CsGAox genes and suggested that protein homology CsGAox are closer to AtGAox than OsGAox. In addition, candidate transcription factors BBR/BPC (BARLEY B RECOMBINANT/BASIC PENTACYSTEINE) and GRAS (GIBBERELLIC ACID-INSENSITIVE, REPRESSOR of GAI, and SCARECROW) which may directly bind promoters of CsGAox genes were predicted. Expression profiles derived from transcriptome data indicated that some CsGAox genes, especially CsGA20ox1, are highly expressed in seedling roots and were down-regulated under GA3 treatment. Ectopic over-expression of CsGA20ox1 in Arabidopsis significantly increased primary root length and lateral root number. Taken together, comprehensive analysis of CsGAoxs would provide a basis for understanding the evolution and function of the CsGAox family.
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DOI：
10.1093/bioinformatics/btr064
发表时间：
2011-04-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Grant CE;Bailey TL;Noble WS
通讯作者：
Noble WS
影响因子：
5.6
作者：
Farrow SC;Facchini PJ
通讯作者：
Facchini PJ
影响因子：
4.9
作者：
FUCHS, E;ATSMON, D;HALEVY, AH
通讯作者：
HALEVY, AH
影响因子：
4.6
作者：
Daviere, Jean-Michel;Achard, Patrick
通讯作者：
Achard, Patrick
影响因子：
8.7
作者：
Chen, Shen;Wang, Xiaojing;Li, Yi
通讯作者：
Li, Yi