MECHANISM OF GLYPHOSATE TOLERANCE IN BIRDSFOOT-TREFOIL (LOTUS-CORNICULATUS)
MECHANISM OF GLYPHOSATE TOLERANCE IN BIRDSFOOT-TREFOIL (LOTUS-CORNICULATUS)
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DOI：
10.1017/s0043174500051304
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发表时间：
1990-11-01
期刊：
影响因子：
2.5
通讯作者：
SOMERS, DA
中科院分区：
文献类型：
--
作者：
BOERBOOM, CM;WYSE, DL;SOMERS, DA
对9个草甘膦耐受性有3倍差异的鸟足三叶草进行了耐草甘膦机制研究。用9个供试材料建立的单茎分株系(营养无性系)在生长室试验中评价了植株对草甘膦的耐受性、喷雾保留率以及对C-14-草甘膦的吸收和转运。温室分株的耐性与大田植株的耐性相关，表明耐性不是植株大小的函数，也不受环境的影响。这9个选择在喷雾保持率和C-14-草甘膦转运方面存在差异，但在草甘膦吸收方面没有差异。保留和转位的差异与草甘膦的耐性水平无关，但可能有助于单个植物的耐受性。测定的9个供试材料中，5-烯醇丙酮基-莽草酸3-磷酸合成酶(EPSPS)的比活力(EC2.5.1.19)变化范围为1.3~3.5nmolmin-1 mg-1蛋白，且与植物耐性呈正相关。这些结果表明，鸟脚三叶草耐草甘膦的主要机制是基于EPSPS活性的水平。
The mechanism of glyphosate tolerance was investigated in nine birdsfoot trefoil selections that exhibited a threefold difference in glyphosate tolerance. Single-stemmed ramets (vegetative clones) established from the nine selections were used to evaluate tolerance to glyphosate, spray retention, and C-14-glyphosate absorption and translocation in growth chamber experiments. The tolerance of greenhouse-grown ramets correlated with the tolerance of field-grown plants, indicating that tolerance was not a function of plant size or affected by environment. The nine selections differed in spray retention and C-14-glyphosate translocation but not in glyphosate absorption. The differences in retention and translocation were not correlated with the level of glyphosate tolerance but could contribute to the tolerance of an individual plant. The specific activity of 5-enolpyruvylshikimate 3-phosphate synthase (EPSPS) (EC 2.5.1.19) ranged from 1.3 to 3.5 nmol min-1 mg-1 protein among the nine selections assayed and was positively correlated with plant tolerance level. These results indicate that the primary mechanism of glyphosate tolerance in birdsfoot trefoil is based on the level of EPSPS activity.