Effects of morphine self-administration on cortical pyramidal cell structure in addiction-prone Lewis rats
Effects of morphine self-administration on cortical pyramidal cell structure in addiction-prone Lewis rats
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DOI：
10.1093/cercor/bhj142
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发表时间：
2007-01-01
期刊：
影响因子：
3.7
通讯作者：
DeFelipe, J.
中科院分区：
文献类型：
--
作者：
Ballesteros-Yanez, I.;Ambrosio, E.;DeFelipe, J.
滥用药物的消费会引起敏感化，产生耐受性、依赖性，最终上瘾。据认为，这些事件的部分原因是药物导致了大脑特定区域神经元回路组织的改变。在本研究中，我们使用细胞内注射荧光黄来检测自我注射吗啡15天后易成瘾的Lewis大鼠皮质锥体神经元可能发生的变化。具体地说，吗啡对初级皮质和运动皮质中第三层锥体神经元的树突的结构、大小和分支复杂性以及棘突密度的影响。我们发现，在吗啡自我注射后，运动皮质中锥体细胞的树突的大小和分支复杂性都有所降低。相反，这些接受吗啡处理的动物的初级锥体神经元有更大更长的树突。此外，吗啡给药大鼠双侧皮质锥体神经元上的脊髓密度较高。这些结果表明，重复服用阿片类药物所产生的行为改变至少有一部分可能归因于锥体细胞结构的改变。
The consumption of drugs of abuse provokes sensitization, the development of tolerance, dependency, and eventually addiction. It is thought that these events are partially a consequence of drug-induced alterations in the organization of neuronal circuits in specific areas of the brain. In the present study, we have used intracellular injections of lucifer yellow to examine the alterations that may occur in cortical pyramidal neurons of addiction-prone Lewis rats following 15 days of self-administration of morphine. Specifically, the effects of morphine on the structure, size and branching complexity of the basal dendrites, and spine density were determined in the basal dendritic arbors of layer III pyramidal neurons in both the prelimbic and motor cortex. We found that following morphine self-administration, there was a reduction in the size and branching complexity of the dendritic arbors of pyramidal cells in the motor cortex. In contrast, prelimbic pyramidal neurons from these morphine-treated animals had larger and longer basal dendritic arbors. Furthermore, the spine density on pyramidal neurons was higher in both cortical regions of morphine self-administered rats. These results suggest that at least part of the behavioral changes produced by repeated opiate administration may be attributed to alterations in pyramidal cell structure.