CEP44 ensures the formation of bona fide centriole wall, a requirement for the centriole-to-centrosome conversion
CEP44 ensures the formation of bona fide centriole wall, a requirement for the centriole-to-centrosome conversion
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DOI：
10.1038/s41467-020-14767-2
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发表时间：
2020-02-14
影响因子：
16.6
通讯作者：
Schiebel, Elmar
中科院分区：
文献类型：
--
作者：
Atorino, Enrico S.;Hata, Shoji;Schiebel, Elmar
中心体是微管组织中具有重要功能的细胞器，每个细胞周期复制一次。中心体复制的第一步是子中心粒的形成，然后是中心粒周围的物质重新聚集到这个中心粒。这个成熟的步骤被称为中心粒到中心体的转化。有人提出，依赖cep295的中心粒周围蛋白募集驱动中心粒转化。在这里，我们显示，基于对促进中心粒生物发生的蛋白质的分析，中心粒结构的发展有助于推动中心粒转化。尽管CEP295与中心粒结合，但与a -微管结合并与POC1B相互作用的管腔中心粒蛋白CEP44的缺失会影响中心粒结构和中心粒转化。POC1B、TUBE1或TUBD1的损伤会扰乱中心粒微管的完整性，也会阻止中心粒到中心体的转化。我们认为，在中心粒管腔和细胞质侧起作用的CEP295、CEP44、POC1B、TUBE1和TUBD1中心粒生物发生途径对中心粒到中心体的转化至关重要。
Centrosomes are essential organelles with functions in microtubule organization that duplicate once per cell cycle. The first step of centrosome duplication is the daughter centriole formation followed by the pericentriolar material recruitment to this centriole. This maturation step was termed centriole-to-centrosome conversion. It was proposed that CEP295-dependent recruitment of pericentriolar proteins drives centriole conversion. Here we show, based on the analysis of proteins that promote centriole biogenesis, that the developing centriole structure helps drive centriole conversion. Depletion of the luminal centriole protein CEP44 that binds to the A-microtubules and interacts with POC1B affecting centriole structure and centriole conversion, despite CEP295 binding to centrioles. Impairment of POC1B, TUBE1 or TUBD1, which disturbs integrity of centriole microtubules, also prevents centriole-to-centrosome conversion. We propose that the CEP295, CEP44, POC1B, TUBE1 and TUBD1 centriole biogenesis pathway that functions in the centriole lumen and on the cytoplasmic side is essential for the centriole-to-centrosome conversion.