MPI tray: a versatile crystallization plate for membrane proteins
MPI tray: a versatile crystallization plate for membrane proteins
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MPI 托盘：用于膜蛋白的多功能结晶板
DOI：
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发表时间：
2017
期刊：
影响因子：
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通讯作者：
Y. Thielmann
中科院分区：
文献类型：
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作者：
Barbara Rathmann;D. Q. Pais;Y. Thielmann
生物大分子的高通量结晶通常是在多孔板上进行的，其设计需要满足不同的、有时甚至是相互冲突的要求。在这方面，由于洗涤剂的普遍使用以及对表面张力的相关影响，处理膜蛋白是一项特别的挑战。本文报道了一种新的结晶板的设计，称为MPI托盘，它是针对膜蛋白样品的紫外和可见光成像而优化的。根据几何和材料方面的基本考虑，对板的表面属性进行了广泛的分析和修改，以改善其在机器人环境中的性能。静电表面电位被认为是影响实验自动设置的主要问题，并发现用乙醇处理结晶板可以有效地消除该电位。
High-throughput crystallization of biological macromolecules is usually performed on multi-well plates, the design of which needs to address different and sometimes conflicting requirements. In this regard, handling of membrane proteins presents a particular challenge owing to the common use of detergents with associated effects on surface tension. Reported here is the design of a new crystallization plate, termed the MPI tray, which is optimized for UV and visible imaging with membrane protein samples. Following basic considerations regarding geometry and material, the surface properties of the plate were subjected to extensive analysis and modification in order to improve the performance in a robotic environment. An electrostatic surface potential was identified as the major problem affecting the automated setup of experiments, and it was found that treatment of the crystallization plate with ethanol is effective in removing this potential.
DOI：
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发表时间：
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期刊：
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影响因子：
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作者：
通讯作者：
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影响因子：
3.8
作者：
Luft, Joseph R.;Wolfley, Jennifer R.;Snell, Edward H.
通讯作者：
Snell, Edward H.