SECONDARY METABOLITES OF AN ENDOPHYTIC FUNGUS Pestalotiopsis uvicola
SECONDARY METABOLITES OF AN ENDOPHYTIC FUNGUS Pestalotiopsis uvicola
复制标题
内生真菌 Pestalotiopsis uvicola 的次级代谢产物
DOI：
10.1007/s10600-017-2112-2
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发表时间：
2017
影响因子：
0.8
通讯作者：
Zhang Xiao-Po
中科院分区：
文献类型：
--
作者：
Qian Yi-Xin;Kang Ji-Chuan;Luo Yi-Kai;He Jun;Wang Lu;Zhang Xiao-Po
Endophytic fungi live within healthy plants without causing any apparent symptoms of disease [1]. They are the focus of potential sources of novel and biologically active natural products [2]. Endophytic Pestalotiopsis species have become a topic of research in many microbial-chemical and pharmacological laboratories because they contain structurally complex and biologically active metabolites [3]. In the course of our previous studies on the bioactivity of endophytic fungi from medicinal plants and their different solvent extracts, some endophytic fungi with various bioactivities have been screened out [4, 5]. As part of our systematic search for new bioactive compounds from endophytic fungi, the endophytic fungus Pestalotiopsis uvicola, isolated from the traditional Chinese medicinal plant Artemisia japonica, was selected for chemical and biological investigations. We have studied the secondary metabolites of this strain and obtained 13 compounds (1–13). The fungal strain P. uvicola GMH31 was isolated from the leaf of A. japonica collected in Guizhou Province, PR China. It was classified as P. uvicola via its morphological characteristics and its rDNA sequence analysis. The strain was deposited in the culture collection of the Engineering and Research Center for Southwest Bio-Pharmaceutical Resources of the National Education Ministry of China, Guizhou University, Guiyang, PR China. The fungus was cultured in 1000 mL Erlenmeyer flasks (u 200) containing 300 mL of liquid Sabouraud broth (SDB) medium at 28qC for 30 days. The culture broth was filtered, and the filtrate was concentrated in vacuum to 5 L and then extracted with EtOAc (10 L) three times. The mycelia were extracted with acetone (5 L) three times, and the acetone solution was concentrated under reduced pressure to afford an aqueous solution (about 1 L). The aqueous solution was extracted with EtOAc (10 L) three times. Both EtOAc solutions from filtrate and mycelia were combined and concentrated under reduced pressure to give a crude extract (30.4 g), which was subjected to column chromatography on silica gel eluting with CHCl
