Transcriptional profiling of ParA and ParB mutants in actively dividing cells of an opportunistic human pathogen Pseudomonas aeruginosa.
Transcriptional profiling of ParA and ParB mutants in actively dividing cells of an opportunistic human pathogen Pseudomonas aeruginosa.
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DOI：
10.1371/journal.pone.0087276
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Jagura-Burdzy G
中科院分区：
文献类型：
--
作者：
Bartosik AA;Glabski K;Jecz P;Mikulska S;Fogtman A;Koblowska M;Jagura-Burdzy G
准确地将染色体分离到子代细胞是确保遗传物质正确遗传的基本过程。在细胞周期简单的细菌中，染色体分离伴随着复制的启动，因为复制的ORIC结构域在形成后不久就开始分离到细胞的相反一半。ParA和PARB蛋白以及特定的DNA序列是分离机制的一部分。铜绿假单胞菌中的PARB和PARB蛋白对于最佳生长、类核分离、细胞分裂和运动是重要的。对位零和PARB缺失突变株与亲本铜绿假单胞菌PAO1161株的比较转录组分析表明，在对数生长的浮游培养物中，基因表达模式发生了全局变化。在两个突变株中受到类似影响的一组基因被指定为PAR调节子，由536个基因组成。PAR调节子包括由两个西格玛因子(RpoN和PvdS)控制的基因以及已知和推定的转录调控因子。在没有PAR蛋白的情况下，诱导了大量来自rpos调节子的基因，反映了减缓细胞生长速度和减速代谢过程的需要。C-di-GMP周转相关基因表达谱的变化表明了这种效应因子在这种信号传递中的作用。所选基因的微阵列数据通过RT-qPCR分析得到确认。将所选基因的启动子区域克隆到无启动子的LacZ基因的上游，并在异源宿主大肠杆菌Δlac中进行分析。对一些被测试的启动子，证实了铜绿假单胞菌PARB和PARB的调控。我们的数据表明，ParA和PARB除了在准确的染色体分离中发挥作用外，还可能作为基因表达的调节器。PAR蛋白是铜绿假单胞菌广泛调控网络的一部分，直接或间接地将染色体分离过程与细胞的生长、分裂和运动联系起来。
Accurate chromosome segregation to progeny cells is a fundamental process ensuring proper inheritance of genetic material. In bacteria with simple cell cycle, chromosome segregation follows replication initiation since duplicated oriC domains start segregating to opposite halves of the cell soon after they are made. ParA and ParB proteins together with specific DNA sequences are parts of the segregation machinery. ParA and ParB proteins in Pseudomonas aeruginosa are important for optimal growth, nucleoid segregation, cell division and motility. Comparative transcriptome analysis of parA null and parB null mutants versus parental P. aeruginosa PAO1161 strain demonstrated global changes in gene expression pattern in logarithmically growing planktonic cultures. The set of genes similarly affected in both mutant strains is designated Par regulon and comprises 536 genes. The Par regulon includes genes controlled by two sigma factors (RpoN and PvdS) as well as known and putative transcriptional regulators. In the absence of Par proteins, a large number of genes from RpoS regulon is induced, reflecting the need for slowing down the cell growth rate and decelerating the metabolic processes. Changes in the expression profiles of genes involved in c-di-GMP turnover point out the role of this effector in such signal transmission. Microarray data for chosen genes were confirmed by RT-qPCR analysis. The promoter regions of selected genes were cloned upstream of the promoter-less lacZ gene and analyzed in the heterologous host E. coliΔlac. Regulation by ParA and ParB of P. aeruginosa was confirmed for some of the tested promoters. Our data demonstrate that ParA and ParB besides their role in accurate chromosome segregation may act as modulators of genes expression. Directly or indirectly, Par proteins are part of the wider regulatory network in P. aeruginosa linking the process of chromosome segregation with the cell growth, division and motility.
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影响因子：
11.4
作者：
Dunham, Thomas D.;Xu, Weijun;Schumacher, Maria A.
通讯作者：
Schumacher, Maria A.
影响因子：
5
作者：
Attila, Can;Ueda, Akihiro;Wood, Thomas K.
通讯作者：
Wood, Thomas K.
影响因子：
3.2
作者：
Bartosik, AA;Lasocki, K;Jagura-Burdzy, G
通讯作者：
Jagura-Burdzy, G
影响因子：
16
作者：
Abel S;Chien P;Wassmann P;Schirmer T;Kaever V;Laub MT;Baker TA;Jenal U
通讯作者：
Jenal U
DOI：
10.1126/science.1188658
发表时间：
2010-06-04
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Christen M;Kulasekara HD;Christen B;Kulasekara BR;Hoffman LR;Miller SI
通讯作者：
Miller SI