Differential RNAs in the sperm cells of asthenozoospermic patients
Differential RNAs in the sperm cells of asthenozoospermic patients
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DOI：
10.1093/humrep/des021
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发表时间：
2012-05-01
影响因子：
6.1
通讯作者：
Oliva, Rafael
中科院分区：
文献类型：
--
作者：
Jodar, Meritxell;Kalko, Susana;Oliva, Rafael
在畸精症患者和其他类型的不育患者中，已经使用微阵列确定了精子中存在的RNA的变化。然而，到目前为止，还没有关于使用微阵列来检测弱精子症患者精子中RNA含量的报道。我们启动了这个项目，目的是与对照组相比，表征弱精子症患者精子细胞中RNA的丰度。为了达到这一目标，我们最初选择了四名正常生育捐赠者和四名弱精子症不育患者。从精子样本中提取等量的RNA，经过不同的质量控制，并与Affymetrix U133 Plus Version 2阵列杂交。与对照组相比，患者中存在几种不同丰度的转录本。随后，我们在更大的样本集合中使用实时荧光PCR验证了三个检测到的转录本(ANXA2、BRD2和OAZ3)的差异表达。这些转录本的表达与进行性运动能力呈正相关。弱精子症患者的精子细胞含有一些改变的RNA，这一点通过微阵列分析和实时荧光定量聚合酶链式反应进行了验证。这些结果为研究这些基因在弱精子症发病机制中的意义以及考虑它们作为不育生物标志物的潜在应用提供了可能性。
Alterations in RNAs present in sperm have been identified using microarrays in teratozoospermic patients and in other types of infertile patients. However, so far, there have been no reports on using microarrays to determine the RNA content of sperm from asthenozoospermic patients.We started the present project with the goal of characterizing the RNA abundance in the sperm cells of asthenozoospermic patients when compared with controls. To reach this objective, we initially selected four normal fertile donors and four asthenozoospermic infertile patients. Equal amounts of RNA were extracted from the sperm samples, subjected to different quality controls and hybridized to the Affymetrix U133 Plus version 2 arrays.Several transcripts were identified that were present in different abundance in patients compared with controls. Subsequently, we validated the differential expression of three of the detected transcripts (ANXA2, BRD2 and OAZ3), using real-time PCR in a larger set of samples. A positive correlation between the expression of these transcripts and progressive motility was observed.The sperm cells of asthenozoospermic patients contain an altered amount of some RNAs as detected using microarray analysis and subsequently validated using real-time PCR. These results open up the possibility to investigate the implication of these genes in the pathogenic mechanisms in asthenozoospermia and to consider their potential utility as infertility biomarkers.