GROWTH-RELATED CHANGES OF OXYGEN-CONSUMPTION RATES OF TUMOR-CELLS GROWN-INVITRO AND INVIVO
GROWTH-RELATED CHANGES OF OXYGEN-CONSUMPTION RATES OF TUMOR-CELLS GROWN-INVITRO AND INVIVO
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DOI：
10.1002/jcp.1041380124
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发表时间：
1989-01-01
影响因子：
5.6
通讯作者：
VAUPEL, P
中科院分区：
文献类型：
--
作者：
KALLINOWSKI, F;TYLER, G;VAUPEL, P
研究了体外或体内生长的肿瘤细胞耗氧率与生长相关的变化。对于体外研究，L929 和 DS-癌肉瘤细胞在人工培养基中培养。为了进行体内研究，将 DS-癌肉瘤细胞植入 Sprague-Dawley 大鼠的腹腔（腹水肿瘤，含有恶性细胞、白细胞、淋巴细胞和巨噬细胞）。通过光度测定法测量摄氧量。在不同生长阶段监测可能影响肿瘤细胞呼吸活动的细胞外介质参数（葡萄糖、乳酸和氨基酸水平、氧和二氧化碳分压以及pH值）。获得的结果清楚地表明，体外生长的肿瘤细胞的摄氧量随着静止期的发展而减少。相反，体内DS-癌肉瘤腹水细胞的呼吸活性随着肿瘤生长达到平台期而增加。观察到的差异不能仅仅归因于监测的环境条件的变化。活化宿主细胞呼吸速率的增加可能会极大地促进体内生长的 DS 癌肉瘤腹水肿瘤呼吸能力的提高。这些数据提供的证据表明，体内实体瘤可以在氧气可用性增强的情况下增加其对氧气的吸收，这不仅是由于氧气供应充足的肿瘤体积增大，而且是由于肿瘤内不同细胞群呼吸活动的升高。
Growth-related changes of oxygen consumption rates of tumor cells, grown in vitro or in vivo, were investigated. For in vitro investigations, L929 and DS-carcinosarcoma cells were cultures in artificial media. For in vivo studies, DS-carcinosarcoma cells were implanted into the abdominal cavity of Sprague-Dawley rats (ascites tumor, containing malignant cells, leukocytes, lymphocytes, and macrophages). Oxygen uptake was measured photometrically. Parameters of the extracellular medium judged to possibly influence the respiratory activity of tumor cells were monitored at different growth stages (glucose, lactate, and amino acid levels, oxygen and carbon dioxide partial pressures, and pH values). The results obtained clearly show that the oxygen uptake of tumor cells grown in vitro decreased as quiescence developed. In contrast, the respiratory activity of in vivo DS-carcinosarcoma ascites cells increased as tumor growth reached plateau phase. The differences observed cannot be attributed solely to changes of the environmental conditions monitored. It is likely that an increased respiration rate of activated host cells might profoundly contribute to the elevation of the respiratory capacity of DS-carcinosarcoma ascites tumors grown in vivo. These data provide evidence that solid tumors in vivo can increase their O2 uptake at an enhanced O2 availability not only due to an enlarged tumor volume with adequate O2 supply but also due to an elevation of the respiratory activity of different cell populations within a tumor.